Molecular cloning and characterization of a novel Dehydrogenase/reductase (SDR family) member 1 gene from human fetal brain

被引：3
作者：
Wu, QH
Xu, M
Cheng, C
Zhou, ZX
Huang, Y
Zhao, W
Zeng, L
Xu, J
Fu, XP
Ying, K
Xie, Y
Mao, YM [1 ]
机构：
[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
[2] United Gene Holdings Ltd, Shanghai 200092, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2001年 / 28卷 / 04期
基金：
中国国家自然科学基金;
关键词：
DHRS1; gene; expression pattern; SDR;
D O I：
10.1023/A:1015722001960
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Short-chain dehydrogenases/reductases (SDR) constitute a large protein family of NAD(P)(H)-dependent oxidoreductase. They are defined by distinct, common sequence motifs and show a wide range of substrate specialisms. By large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a novel human SDR-type dehydrogenase/reductase gene named Dehydrogenase/reductase (SDR family) member 1 (DHRS1). The DHRS1 cDNA is 1411 base pair in length, encoding a 314-amino-acid polypeptide which has a SDR motif. Northern blot reveals two bands, of about 0.9 and 1.4 kb in size. These two forms are expressed in many tissues. The DHRS1 gene is localized on chromosome 14q21.3. It has 9 exons and spans 9.2 kb of the genomic DNA.
引用
收藏
页码：193 / 198
页数：6
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