Fusobacterium necrophorum haemolysin stimulates motility of ileal longitudinal smooth muscle of the guinea-pig

被引:1
作者
Kanoe, M
Toyoda, Y
Shibata, H
Nasu, T [1 ]
机构
[1] Yamaguchi Univ, Fac Agr, Dept Vet Pharmacol, Yamaguchi 753, Japan
[2] Tokyo Univ Agr & Technol, Lab Anim Sci, Tokyo, Japan
[3] Yamaguchi Univ, Fac Agr, Dept Vet Microbiol, Yamaguchi 753, Japan
关键词
Fusobacterium necrophorum; haemolysin; calcium; intestine; smooth muscle;
D O I
10.1111/j.1472-8206.1999.tb00360.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Fusobacterium necrophorum haemolysin (0.5-3.1 mg protein/mL) dose-dependently induced contractions of the isolated ileal longitudinal smooth muscle of the guinea-pig. The haemolysin (3.1 mg protein/mL)-induced maximum contraction of 75% of the response to 60 mM K+ declined within 17 min and the muscles then demonstrated rhythmic contractions. Tetrodotoxin (3.1 x 10(-6) M) had no effect on the contraction due to the haemolysin. After incubation in Ca2+-free medium, the ileal response to the haemolysin was lost. Verapamil, a Ca2+ channel blocker, dose-dependently inhibited the contraction to the haemolysin. The rabbit anti-serum against F. necrophorum haemolysin inhibited the haemolysin-induced contraction of ileal muscle. The bacterial haemagglutinin and the lipopolysaccharide had no effect on the response of ileal muscle. These findings suggest that the haemolysin-induced direct stimulation of ileal motility dependant on Ca2+ influx will increase the probability of contact of F. necrophorum and ileal mucosa and could increase the chances of colonization for F. necrophorum. (C) 1999 Editions scientifiques et medicales Elsevier SAS.
引用
收藏
页码:547 / 554
页数:8
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