Vitamin D metabolite profiling using liquid chromatography-tandem mass spectrometry (LC-MS/MS)

被引：45

作者：

Jones, Glenville ^{[1
]}

Kaufmann, Martin ^{[1
]}

机构：

[1] Queens Univ, Dept Biomed & Mol Sci, Botterell Hall,Room 650, Kingston, ON K7L 3N6, Canada

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2016年 / 164卷

基金：

加拿大健康研究院;

关键词：

Metabolomics; 25-OH-D-3; Vitamin D assay; LC-MS/MS; DMEQ-TAD; PROTEIN-BINDING RADIOASSAY; 24,25-DIHYDROXYVITAMIN D; 25-HYDROXYVITAMIN D; HUMAN-PLASMA; CYP24A1; SERUM; DERIVATIZATION; MUTATION; INFANTS; ASSAY;

D O I：

10.1016/j.jsbmb.2015.09.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography tandem mass spectrometry (LC-MS/MS) has emerged as the latest technology to be used to assay the metabolites of vitamin D. The method uses molecular mass as a detection technique after straightforward extraction and chromatography steps. LC-MS/MS assay provides a level of accuracy and reproducibility not seen before with other methods and is beginning to rival antibody-based methods in terms of sensitivity and convenience. Methods for detection of underivatized and DMEQ-TAD derivatized vitamin D metabolites are evaluated. Sensitivity is improved by 10-100 fold with derivatization and allows for the simultaneous assay of multiple vitamin D metabolites, a process termed vitamin D metabolite profiling. Clinical and research applications of vitamin D metabolite profiling are discussed. Published by Elsevier Ltd.

引用

页码：110 / 114

页数：5

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