Vitamin D metabolite profiling using liquid chromatography-tandem mass spectrometry (LC-MS/MS)

被引：45

作者：

Jones, Glenville ^{[1
]}

Kaufmann, Martin ^{[1
]}

机构：

[1] Queens Univ, Dept Biomed & Mol Sci, Botterell Hall,Room 650, Kingston, ON K7L 3N6, Canada

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2016年 / 164卷

基金：

加拿大健康研究院;

关键词：

Metabolomics; 25-OH-D-3; Vitamin D assay; LC-MS/MS; DMEQ-TAD; PROTEIN-BINDING RADIOASSAY; 24,25-DIHYDROXYVITAMIN D; 25-HYDROXYVITAMIN D; HUMAN-PLASMA; CYP24A1; SERUM; DERIVATIZATION; MUTATION; INFANTS; ASSAY;

D O I：

10.1016/j.jsbmb.2015.09.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liquid chromatography tandem mass spectrometry (LC-MS/MS) has emerged as the latest technology to be used to assay the metabolites of vitamin D. The method uses molecular mass as a detection technique after straightforward extraction and chromatography steps. LC-MS/MS assay provides a level of accuracy and reproducibility not seen before with other methods and is beginning to rival antibody-based methods in terms of sensitivity and convenience. Methods for detection of underivatized and DMEQ-TAD derivatized vitamin D metabolites are evaluated. Sensitivity is improved by 10-100 fold with derivatization and allows for the simultaneous assay of multiple vitamin D metabolites, a process termed vitamin D metabolite profiling. Clinical and research applications of vitamin D metabolite profiling are discussed. Published by Elsevier Ltd.

引用

页码：110 / 114

页数：5

共 31 条

[1]

Armas LAG, 2014, 17 VIT D WORKSH CHIC

[2] Metabolic profiling of major vitamin D metabolites using Diels-Alder derivatization and ultra-performance liquid chromatography-tandem mass spectrometry [J].

Aronov, Pavel A. ;

Hall, Laura M. ;

Dettmer, Katja ;

Stephensen, Charles B. ;

Hammock, Bruce D. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 391 (05) :1917-1930

[3] Development and comparison of three liquid chromatography-atmospheric pressure chemical ionization/mass spectrometry methods for determining vitamin D metabolites in human serum [J].

Bedner, Mary ;

Phinney, Karen W. .

JOURNAL OF CHROMATOGRAPHY A, 2012, 1240 :132-139

[4] COMPETITIVE BINDING ASSAY FOR VITAMIN-D AND 25-OH VITAMIN-D [J].

BELSEY, R ;

DELUCA, HF ;

POTTS, JT .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1971, 33 (03) :554-+

[5] Significance of Serum 24,25-Dihydroxyvitamin D in the Assessment of Vitamin D Status: A Double-edged Sword? [J].

Cashman, Kevin D. ;

Hayes, Aoife ;

Galvin, Karen ;

Merkel, Joyce ;

Jones, Glenville ;

Kaufmann, Martin ;

Hoofnagle, Andrew N. ;

Carter, Graham D. ;

Durazo-Arvizu, Ramon A. ;

Sempos, Christopher T. .

CLINICAL CHEMISTRY, 2015, 61 (04) :636-645

[6] SENSITIVE, PRECISE, AND CONVENIENT METHOD FOR DETERMINATION OF 1,25-DIHYDROXYVITAMIN-D IN HUMAN-PLASMA [J].

EISMAN, JA ;

HAMSTRA, AJ ;

KREAM, BE ;

DELUCA, HF .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1976, 176 (01) :235-243

[7] COMPETITIVE PROTEIN-BINDING RADIOASSAY FOR 25-HYDROXYCHOLECALCIFEROL [J].

HADDAD, JG .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1971, 33 (06) :992-+

[8]

HOLLIS BW, 1985, CLIN CHEM, V31, P1815

[9] 25-OHD3-26,23-LACTONE - METABOLITE OF VITAMIN-D3 THAT IS 5 TIMES MORE POTENT THAN 25-OHD3 IN THE RAT PLASMA COMPETITIVE PROTEIN-BINDING RADIOASSAY [J].

HORST, RL .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1979, 89 (01) :286-293

[10] DETERMINATION OF THE VITAMIN-D METABOLITES ON A SINGLE PLASMA SAMPLE - CHANGES DURING PARTURITION IN DAIRY-COWS [J].

HORST, RL ;

SHEPARD, RM ;

JORGENSEN, NA ;

DELUCA, HF .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 192 (02) :512-523

← 1 2 3 4 →