Modulatory effects of hypocretin-1/orexin-A with glutamate and γ-aminobutyric acid on freshly isolated pyramidal neurons from the rat prefrontal cortex

被引：20
作者：
Song, Cheng-hui
Chen, Xiao-wei
Xia, Jian-xia
Yu, Zheng-ping
Hu, Zhi-an [1 ]
机构：
[1] Third Mil Med Univ, Dept Neurobiol, Chongqing 40038, Peoples R China
[2] Third Mil Med Univ, Dept Occupat Hlth, Chongqing 40038, Peoples R China
来源：
NEUROSCIENCE LETTERS | 2006年 / 399卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
hypocretins; glutamate; gamma-aminobutyric acid; prefrontal cortex; pyramidal neurons; wakefulness;
D O I：
10.1016/j.neulet.2006.01.065
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
It is widely known that hypocretins are essential for the regulation of wakefulness. Our recent reports have found that hypocretin-1 shows a direct postsynaptic excitatory effect on rat prefrontal cortex (PFC) pyramidal neurons. It remains unclear whether hypocretin-1 may interact with two classical neurotransmitter systems, glutamate and gamma-aminobutyric acid (GABA) in rat PFC. For this reason, we here investigated the modulatory actions of hypocretin-1 with these two transmitters on freshly isolated PFC pyramidal neurons using whole-cell patch-clamp recordings. We found that coadministration of hypocretin-1 and glutamate showed a synergistic effect on the recorded cells, and hypocretin-1 could excite the neurons even if GABA was present. Thus, our data suggest that there may be hypocretin-glutamate and hypocretin-GABA interactions in the PFC. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：101 / 105
页数：5
相关论文
共 37 条
[1]   Exclusive postsynaptic action of hypocretin-orexin on sublayer 6b cortical neurons [J].
Bayer, L ;
Serafin, M ;
Eggermann, E ;
Saint-Mleux, B ;
Marchard, D ;
Jones, BE ;
Mühlethaler, M .
JOURNAL OF NEUROSCIENCE, 2004, 24 (30) :6760-6764
[2]   What keeps us awake: The neuropharmacology of stimulants and wakefulness promoting medications [J].
Boutrel, B ;
Koob, GF .
SLEEP, 2004, 27 (06) :1181-1194
[3]   GABA-A AND GABA-B RECEPTOR-SITE DISTRIBUTION IN THE RAT CENTRAL-NERVOUS-SYSTEM [J].
BOWERY, NG ;
HUDSON, AL ;
PRICE, GW .
NEUROSCIENCE, 1987, 20 (02) :365-383
[4]  
Cai X, 2002, J PHYSIOL-LONDON, V540, P743
[5]   Narcolepsy in orexin knockout mice:: Molecular genetics of sleep regulation [J].
Chemelli, RM ;
Willie, JT ;
Sinton, CM ;
Elmquist, JK ;
Scammell, T ;
Lee, C ;
Richardson, JA ;
Williams, SC ;
Xiong, YM ;
Kisanuki, Y ;
Fitch, TE ;
Nakazato, M ;
Hammer, RE ;
Saper, CB ;
Yanagisawa, M .
CELL, 1999, 98 (04) :437-451
[6]   The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity [J].
De Lecea, L ;
Kilduff, TS ;
Peyron, C ;
Gao, XB ;
Foye, PE ;
Danielson, PE ;
Fukuhara, C ;
Battenberg, ELF ;
Gautvik, VT ;
Bartlett, FS ;
Frankel, WN ;
van den Pol, AN ;
Bloom, FE ;
Gautvik, KM ;
Sutcliffe, JG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (01) :322-327
[7]   Behavioral arousal and increased dopamine efflux after blockade of NMDA-receptors in the prefrontal cortex are dependent on activation of glutamatergic neurotransmission [J].
Feenstra, MGP ;
Botterblom, MHA ;
van Uum, JFM .
NEUROPHARMACOLOGY, 2002, 42 (06) :752-763
[8]   Serotonin receptors modulate GABAA receptor channels through activation of anchored protein kinase C in prefrontal cortical neurons [J].
Feng, J ;
Cai, X ;
Zhao, JH ;
Yan, Z .
JOURNAL OF NEUROSCIENCE, 2001, 21 (17) :6502-6511
[9]   CELLULAR BASIS OF WORKING-MEMORY [J].
GOLDMANRAKIC, PS .
NEURON, 1995, 14 (03) :477-485
[10]   Hypocretin (orexin) induces calcium transients in single spines postsynaptic to identified thalamocortical boutons in prefrontal slice [J].
Lambe, EK ;
Aghajanian, GK .
NEURON, 2003, 40 (01) :139-150
1234