Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans

被引：807

作者：

Mateus, Jose ^{[1
]}

Grifoni, Alba ^{[1
]}

Tarke, Alison ^{[1
]}

Sidney, John ^{[1
]}

Ramirez, Sydney, I ^{[1
,3
]}

Dan, Jennifer M. ^{[1
,3
]}

Burger, Zoe C. ^{[3
]}

Rawlings, Stephen A. ^{[3
]}

Smith, Davey M. ^{[3
]}

Phillips, Elizabeth ^{[2
]}

Mallal, Simon ^{[2
]}

Lammers, Marshall ^{[1
]}

Rubiro, Paul ^{[1
]}

Quiambao, Lorenzo ^{[1
]}

Sutherland, Aaron ^{[1
]}

Yu, Esther Dawen ^{[1
]}

Antunes, Ricardo da Silva ^{[1
]}

Greenbaum, Jason ^{[1
]}

Frazier, April ^{[1
]}

Markmann, Alena J. ^{[4
]}

Premkumar, Lakshmanane ^{[5
]}

de Silva, Aravinda ^{[5
]}

Peters, Bjoern ^{[1
,3
]}

Crotty, Shane ^{[1
,3
]}

Sette, Alessandro ^{[1
,3
]}

Weiskopf, Daniela ^{[1
]}

机构：

[1] La Jolla Inst Immunol, Ctr Infect Dis & Vaccine Res, La Jolla, CA 92037 USA

[2] Murdoch Univ, Inst Immunol & Infect Dis, Perth, WA 6150, Australia

[3] Univ Calif San Diego, Dept Med, Div Infect Dis & Global Publ Hlth, La Jolla, CA 92037 USA

[4] Univ N Carolina, Dept Med, Div Infect Dis, Sch Med, Chapel Hill, NC 27599 USA

[5] Univ N Carolina, Dept Microbiol & Immunol, Sch Med, Chapel Hill, NC 27599 USA

来源：

SCIENCE | 2020年 / 370卷 / 6512期

关键词：

FOLLICULAR HELPER-CELLS; HUMAN CORONAVIRUSES; RECOGNITION;

D O I：

10.1126/science.abd3871

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many unknowns exist about human immune responses to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. SARS-CoV-2-reactive CD4(+) T cells have been reported in unexposed individuals, suggesting preexisting cross-reactive T cell memory in 20 to 50% of people. However, the source of those T cells has been speculative. Using human blood samples derived before the SARS-CoV-2 virus was discovered in 2019, we mapped 142 T cell epitopes across the SARS-CoV-2 genome to facilitate precise interrogation of the SARS-CoV-2-specific CD4(+) T cell repertoire. We demonstrate a range of preexisting memory CD4(+) T cells that are cross-reactive with comparable affinity to SARS-CoV-2 and the common cold coronaviruses human coronavirus (HCoV)-OC43, HCoV-229E, HCoV-NL63, and HCoV-HKU1. Thus, variegated T cell memory to coronaviruses that cause the common cold may underlie at least some of the extensive heterogeneity observed in coronavirus disease 2019 (COVID-19) disease.

引用

页码：89 / +

页数：47

共 51 条

[1]

Alba Grifoni, 2020, BIORXIV PREPRINT, DOI [10.1101/2020.02.12.946087, DOI 10.1101/2020.02.12.946087]

[2]

Amanat F, 2020, NAT MED, V26, P1033, DOI [10.1038/s41591-020-0913-5, 10.1101/2020.03.17.20037713]

[3] Definition of Human Epitopes Recognized in Tetanus Toxoid and Development of an Assay Strategy to Detect Ex Vivo Tetanus CD4+ T Cell Responses [J].

Antunes, Ricardo da Silva ;

Paul, Sinu ;

Sidney, John ;

Weiskopf, Daniela ;

Dan, Jennifer M. ;

Phillips, Elizabeth ;

Mallal, Simon ;

Crotty, Shane ;

Sette, Alessandro ;

Arlehamn, Cecilia S. Lindestam .

PLOS ONE, 2017, 12 (01)

[4] A Quantitative Analysis of Complexity of Human Pathogen-Specific CD4 T Cell Responses in Healthy M. tuberculosis Infected South Africans [J].

Arlehamn, Cecilia S. Lindestam ;

McKinney, Denise M. ;

Carpenter, Chelsea ;

Paul, Sinu ;

Rozot, Virginie ;

Makgotlho, Edward ;

Gregg, Yolande ;

van Rooyen, Michele ;

Ernst, Joel D. ;

Hatherill, Mark ;

Hanekom, Willem A. ;

Peters, Bjoern ;

Scriba, Thomas J. ;

Sette, Alessandro .

PLOS PATHOGENS, 2016, 12 (07)

[5] Th1 versus Th2 T cell polarization by whole-cell and acellular childhood pertussis vaccines persists upon re-immunization in adolescence and adulthood [J].

Bancroft, Tara ;

Dillon, Myles B. C. ;

Antunes, Ricardo da Silva ;

Paul, Sinu ;

Peters, Bjoern ;

Crotty, Shane ;

Arlehamn, Cecilia S. Lindestam ;

Sette, Alessandro .

CELLULAR IMMUNOLOGY, 2016, 304 :35-43

[6]

Braun Julian., 2020, medRxiv, DOI [10.1101/2020.04.17.20061440, DOI 10.1101/2020.04.17.20061440]

[7] Automatic Generation of Validated Specific Epitope Sets [J].

Carrasco Pro, Sebastian ;

Sidney, John ;

Paul, Sinu ;

Arlehamn, Cecilia Lindestam ;

Weiskopf, Daniela ;

Peters, Bjoern ;

Sette, Alessandro .

JOURNAL OF IMMUNOLOGY RESEARCH, 2015, 2015

[8] T cell receptor recognition of MHC class II-bound peptide flanking residues enhances immunogenicity and results in altered TCR V region usage [J].

Carson, RT ;

Vignali, KM ;

Woodland, DL ;

Vignali, DAA .

IMMUNITY, 1997, 7 (03) :387-399

[9]

-Cervantes L. Kuri, 2020, 2020051841011 BIORXI

[10] A Cytokine-Independent Approach To Identify Antigen-Specific Human Germinal Center T Follicular Helper Cells and Rare Antigen-Specific CD4+ T Cells in Blood [J].

Dan, Jennifer M. ;

Arlehamn, Cecilia S. Lindestam ;

Weiskopf, Daniela ;

Antunes, Ricardo da Silva ;

Havenar-Daughton, Colin ;

Reiss, Samantha M. ;

Brigger, Matthew ;

Bothwell, Marcella ;

Sette, Alessandro ;

Crotty, Shane .

JOURNAL OF IMMUNOLOGY, 2016, 197 (03) :983-993

← 1 2 3 4 5 6 →