KPC-50 Confers Resistance to Ceftazidime-Avibactam Associated with Reduced Carbapenemase Activity

被引：44

作者：

Poirel, Laurent ^{[1
,2
,3
,4
,5
]}

Vuillemin, Xavier ^{[1
,2
]}

Juhas, Mario ^{[1
,2
]}

Masseron, Amandine ^{[1
,2
]}

Bechtel-Grosch, Ursina ^{[6
]}

Tiziani, Simon ^{[7
]}

Mancini, Stefano ^{[8
]}

Nordmann, Patrice ^{[1
,2
,3
,4
,5
,9
,10
]}

机构：

[1] Univ Fribourg, Fac Sci & Med, Emerging Antibiot Resistance Unit, Fribourg, Switzerland

[2] Univ Fribourg, Fac Sci & Med, Med & Mol Microbiol, Fribourg, Switzerland

[3] Univ Fribourg, Swiss Natl Reference Ctr Emerging Antibiot Resist, Fribourg, Switzerland

[4] INSERM, European Unit, IAME, Paris, France

[5] Univ Fribourg, Fribourg, Switzerland

[6] Univ Hosp Zurich, Dept Trauma Surg, Zurich, Switzerland

[7] Univ Zurich, Univ Hosp Zurich, Inst Intens Med, Zurich, Switzerland

[8] Univ Zurich, Inst Med Microbiol, Zurich, Switzerland

[9] Univ Hosp Ctr, Inst Microbiol, Lausanne, Switzerland

[10] Univ Lausanne, Lausanne, Switzerland

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2020年 / 64卷 / 08期

基金：

瑞士国家科学基金会;

关键词：

KPC; ceftazidime-avibactam; Klebsiella pneumoniae; KLEBSIELLA-PNEUMONIAE; LACTAMASE;

D O I：

10.1128/AAC.00321-20

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

KPC-50 is a KPC-3 variant identified from a Klebsiella pneumoniae clinical isolate recovered in Switzerland in 2019. Compared to KPC-3, KPC-50 shows (i) a three-amino-acid insertion (Glu-Ala-Val) between amino acids 276 and 277, (ii) an increased affinity to ceftazidime, (iii) a decreased sensitivity to avibactam, explaining the ceftazidime-avibactam resistance, and (iv) an association with a sharp reduction of its carbapenemase activity.

引用

页数：5

共 23 条

[1] Meropenem-Vaborbactam as Salvage Therapy for Ceftazidime-Avibactam-Resistant Klebsiella pneumoniae Bacteremia and Abscess in a Liver Transplant Recipient [J].

Athans, Vasilios ;

Neuner, Elizabeth A. ;

Hassouna, Habiba ;

Richter, Sandra S. ;

Keller, George ;

Castanheira, Mariana ;

Brizendine, Kyle D. ;

Mathers, Amy J. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2019, 63 (01)

[2] Klebsiella pneumoniae Carbapenemase-2 (KPC-2), Substitutions at Ambler Position Asp179, and Resistance to Ceftazidime-Avibactam: Unique Antibiotic-Resistant Phenotypes Emerge from β-Lactamase Protein Engineering [J].

Barnes, Melissa D. ;

Winkler, Marisa L. ;

Taracila, Magdalena A. ;

Page, Malcolm G. ;

Desarbre, Eric ;

Kreiswirth, Barry N. ;

Shields, Ryan K. ;

Minh-Hong Nguyen ;

Clancy, Cornelius ;

Spellberg, Brad ;

Papp-Wallace, Krisztina M. ;

Bonomo, Robert A. .

MBIO, 2017, 8 (05)

[3] Identification of plasmids by PCR-based replicon typing [J].

Carattoli, A ;

Bertini, A ;

Villa, L ;

Falbo, V ;

Hopkins, KL ;

Threlfall, EJ .

JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 63 (03) :219-228

[4]

Compain F, 2017, ANTIMICROB AGENTS CH, V61, DOI [10.1128/AAC.00451-17, 10.1128/aac.00451-17]

[5] Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates [J].

Diancourt, L ;

Passet, V ;

Verhoef, J ;

Grimont, PAD ;

Brisse, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4178-4182

[6]

EUCAST, 2020, BREAKP TABL INT MICS

[7] Genomic characterization of a KPC-23-producing Klebsiella pneumoniae ST258 clinical isolate resistant to ceftazidime-avibactam [J].

Galani, I. ;

Antoniadou, A. ;

Karaiskos, I. ;

Kontopoulou, K. ;

Giamarellou, H. ;

Souli, M. .

CLINICAL MICROBIOLOGY AND INFECTION, 2019, 25 (06) :763.e5-763.e8

[8] Nosocomial spread of the integron-located veb-1-like cassette encoding an extended-spectrum β-lactamase in Pseudomonas aeruginosa in Thailand [J].

Girlich, D ;

Naas, T ;

Leelaporn, A ;

Poirel, L ;

Fennewald, M ;

Nordmann, P .

CLINICAL INFECTIOUS DISEASES, 2002, 34 (05) :603-611

[9] Mutations in blaKPC-3 That Confer Ceftazidime-Avibactam Resistance Encode Novel KPC-3 Variants That Function as Extended-Spectrum β-Lactamases [J].

Haidar, Ghady ;

Clancy, Cornelius J. ;

Shields, Ryan K. ;

Hao, Binghua ;

Cheng, Shaoji ;

Nguyen, M. Hong .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2017, 61 (05)

[10] Ceftazidime/avibactam resistance associated with L169P mutation in the omega loop of KPC-2 [J].

Hemarajata, Peera ;

Humphries, Romney M. .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2019, 74 (05) :1241-1243

← 1 2 3 →