Structural changes of rabbit myosin subfragment 1 altered by malonaldehyde, a byproduct of lipid oxidation

被引：36

作者：

Li, SJ ^{[1
]}

King, AJ ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 08期

关键词：

rabbit myosin subfragment 1; malonaldehyde; secondary structure;

D O I：

10.1021/jf990028y

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Rabbit myosin subfragment 1 was allowed to react with malonaldehyde, a byproduct of lipid oxidation in a model system containing the subfragment 1 and malonaldehyde. The modified subfragment 1 was compared to the control, with use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), circular dichorism (CD), Fourier transform infrared spectroscopy (FT-IR), and free amino group measurements. Both the modified and control samples were cleaved with cyanogen bromide (BrCN) treatment, and the BrCN fragments were analyzed by SDS-PAGE with tricine gel. Results from SDS-PACE suggest that malonaldehyde causes cross-linking or polymerization of the protein during incubation. Malonaldehyde also reduced cr-helix content (CD), increased random structure (PT-IR), and eliminated some beta-strand structure (FT-IR) in subfragment 1.

引用

页码：3124 / 3129

页数：6

共 29 条

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