Structural changes of rabbit myosin subfragment 1 altered by malonaldehyde, a byproduct of lipid oxidation

被引：36
作者：
Li, SJ [1 ]
King, AJ [1 ]
机构：
[1] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1999年 / 47卷 / 08期
关键词：
rabbit myosin subfragment 1; malonaldehyde; secondary structure;
D O I：
10.1021/jf990028y
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Rabbit myosin subfragment 1 was allowed to react with malonaldehyde, a byproduct of lipid oxidation in a model system containing the subfragment 1 and malonaldehyde. The modified subfragment 1 was compared to the control, with use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), circular dichorism (CD), Fourier transform infrared spectroscopy (FT-IR), and free amino group measurements. Both the modified and control samples were cleaved with cyanogen bromide (BrCN) treatment, and the BrCN fragments were analyzed by SDS-PAGE with tricine gel. Results from SDS-PACE suggest that malonaldehyde causes cross-linking or polymerization of the protein during incubation. Malonaldehyde also reduced cr-helix content (CD), increased random structure (PT-IR), and eliminated some beta-strand structure (FT-IR) in subfragment 1.
引用
收藏
页码：3124 / 3129
页数：6
相关论文
共 29 条
[1]   REACTION OF AN AUTOXIDIZED LIPID WITH PROTEINS [J].
ANDREWS, F ;
BJORKSTEN, J ;
TRENK, FB ;
HENICK, AS ;
KOCH, RB .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1965, 42 (09) :779-+
[2]  
[Anonymous], 1999, QUALITY ATTRIBUTES M
[3]  
[Anonymous], FUNCTIONAL PROPERTIE
[4]   EXTRACTION AND PARTIAL CHARACTERIZATION OF DIALYZABLE PRODUCTS ORIGINATING FROM THE PEROXIDATION OF LIVER MICROSOMAL LIPIDS AND INHIBITING MICROSOMAL GLUCOSE 6-PHOSPHATASE ACTIVITY [J].
BENEDETTI, A ;
CASINI, AF ;
FERRALI, M ;
COMPORTI, M .
BIOCHEMICAL PHARMACOLOGY, 1979, 28 (19) :2909-2918
[5]   Molecular and microstructural studies of thermal denaturation and gelation of beta-lactoglobulins A and B [J].
Boye, JI ;
Ma, CY ;
Ismail, A ;
Harwalkar, VR ;
Kalab, M .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (05) :1608-1618
[6]   REACTION OF MYOSIN WITH MALONALDEHYDE [J].
BUTTKUS, H .
JOURNAL OF FOOD SCIENCE, 1967, 32 (04) :432-&
[7]   PROTECTIVE EFFECT OF LIPIDIC SURFACES AGAINST PRESSURE-INDUCED CONFORMATIONAL-CHANGES OF POLY(L-LYSINE) [J].
CARRIER, D ;
MANTSCH, HH ;
WONG, PTT .
BIOCHEMISTRY, 1990, 29 (01) :254-258
[8]   CHEMICAL, PHYSICAL, AND FUNCTIONAL-PROPERTIES OF OXIDIZED TURKEY WHITE MUSCLE MYOFIBRILLAR PROTEINS [J].
DECKER, EA ;
XIONG, YL ;
CALVERT, JT ;
CRUM, AD ;
BLANCHARD, SP .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (02) :186-189
[9]   CHEMISTRY AND BIOCHEMISTRY OF 4-HYDROXYNONENAL, MALONALDEHYDE AND RELATED ALDEHYDES [J].
ESTERBAUER, H ;
SCHAUR, RJ ;
ZOLLNER, H .
FREE RADICAL BIOLOGY AND MEDICINE, 1991, 11 (01) :81-128
[10]  
ESTERBAUER H, 1987, LIPID PEROXIDATION C, V44, P45
123