Protective effect of lycopene on fluoride-induced ameloblasts apoptosis and dental fluorosis through oxidative stress-mediated Caspase pathways

被引:75
作者
Li, Weishan [1 ]
Jiang, Binghua [2 ]
Cao, Xianglin [2 ]
Xie, Yongjiang [3 ]
Huang, Ting [1 ]
机构
[1] Jiamusi Univ, Affiliated Stomatol Hosp, Jiamusi 154007, Peoples R China
[2] Hainan Stomatol Hosp, Haikou 570105, Peoples R China
[3] Daqing Oilfield Gen Hosp, Daqing 163000, Peoples R China
关键词
Lycopene; Fluoride; Oxidative stress; Rat; SODIUM-FLUORIDE; ENAMEL ORGAN; DNA-DAMAGE; CELL-CYCLE; RATS; JNK; MITOCHONDRIA; ASSOCIATION; ANTIOXIDANT; ACTIVATION;
D O I
10.1016/j.cbi.2016.11.021
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fluoride is an environmental toxicant and induces dental fluorosis and oxidative stress. Lycopene (LYC) is an effective antioxidant that is reported to attenuate fluoride toxicity. To determine the effects of LYC on sodium fluoride (NaF) -induced teeth and ameloblasts toxicity, rats were treated with NaF (10 mg/kg) and/or LYC (10 mg/kg) by orally administration for 5 weeks; ameloblasts were treated with NaF (5 mM) and/or LYC (2 mu M) for 6 h. We found that the concentrations of fluoride, malondialdehyde (MDA) and reactive oxygen species (ROS), gene expressions and activities of Caspase-9 and Caspase-3, and the gene expressions of Bax were significantly decreased, while the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX), the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated rats group; concentrations of MDA and ROS, gene expressions and activities of Caspase-9 and Caspase-3, and the gene expression of Bax, and ameloblasts apoptosis rate were significantly decreased, while the activities of SOD and GPX, the gene expression of Bcl-2 were significantly increased in the LYC + NaF-treated ameloblasts group. These results suggest that LYC significantly combated NaF-induced ameloblasts apoptosis and dental fluorosis by attenuation oxidative stress and down-regulation Caspase pathway. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:27 / 34
页数:8
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