LC-MS/MS determination of the HIV-1 protease inhibitor indinavir in brain and testis of mice

被引：4
作者：
de Oliveira, MP
Venisse, N
Couet, W
Olivier, JC
机构：
[1] Fac Med & Pharm, EA Pharmacol Medicaments Anti Infectieux 3809, F-86022 Poitiers, France
[2] Ctr Hosp Univ Poitiers, Lab Pharmacocinet, Fac Med & Pharm, F-86022 Poitiers, France
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 40卷 / 02期
关键词：
indinavir; amprenavir; LC-MS/MS; solid-phase extraction; brain; testis;
D O I：
10.1016/j.jpba.2005.07.008
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A rapid and sensitive method for the determination of indinavir in mice brain and testis is described and validation data are provided. Indinavir and the internal standard (IS) amprenavir were isolated from homogenized tissue matrices using a mixed-mode solid-phase extraction (SPE) procedure and were then analyzed by reversed-phase liquid chromatography/tandem mass spectrometry (LC-MS/MS). The mass spectrometer in the positive-ion multiple reaction monitoring mode used pairs of ions at m/z of 614.1/421.3 for indinavir and of 506.1/245.3 for IS. The calibration curves were linear over the range 0.0012-0.0390 mu mol/kg for brain and 0.39-12.50 mu mol/kg for testis. Linearity, repeatability and accuracy were validated. The applicability of the method was demonstrated by assessing indinavir in brain and testis of three mice dosed with intravenous bolus administration of indinavir (16.3 mu mol/ka). (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：353 / 359
页数：7
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