Expressions of Shell Matrix Protein Genes in the Pearl Sac and Its Correlation with Pearl Weight in the First 6Months of Pearl Formation in Hyriopsis cumingii

被引:20
作者
Jin, Can [1 ]
Zhao, Jing-Ying [2 ]
Liu, Xiao-Jun [1 ,3 ]
Li, Jia-Le [1 ,3 ,4 ]
机构
[1] Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China
[2] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Biol Sci 2016, Class 1, Shanghai 201306, Peoples R China
[3] Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
[4] Shanghai Engn Res Ctr Aquaculture, Shanghai 201306, Peoples R China
基金
中国国家自然科学基金;
关键词
Pearl formation; Nacre; Matrix protein; Pearl weight; Hyriopsis cumingii; NACREOUS LAYER; PINCTADA-MARGARITIFERA; CARBONIC-ANHYDRASE; OYSTER; QUALITY; BIOMINERALIZATION; IDENTIFICATION; MUSSEL; DEPOSITION; TRAITS;
D O I
10.1007/s10126-019-09876-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Matrix proteins regulate crystal nucleation, morphology, and polymorphism during pearl biomineralization and have significant correlations with pearl quality traits in nucleated pearls. However, there is little information about the connection between pearl quality traits and matrix proteins in non-nucleated pearls. In this study, we analyzed CaCO3 deposition during the first month of non-nucleated pearl formation and examined the expression patterns of ten shell matrix protein genes (Hcperlucin, hic31, silkmapin, hic22, hic74, hic52, HcTyr, HcCA3, hic24 and Hc-upsalin) in the pearl sac of Hyriopsis cumingii. During pearl formation, CaCO3 crystals were initially deposited in a disorderly manner during days 12 and 15 of pearl formation. On days 18 and 21, CaCO3 crystals gradually nucleated on an organic membrane, and the pattern of crystal deposition changed markedly. Between days 24 and 30, crystals similar to nacre tablets were deposited; they then grew and formed connections in a more orderly fashion, eventually forming the nacreous layer. We observed high expression levels of shell matrix proteins during the phases of disordered or ordered CaCO3 deposition, suggesting they were involved in non-nucleated pearl formation. Furthermore, the expressions of nine matrix proteins were significantly correlated with pearl weight during the first 6months after grafting. The prismatic-layer matrix protein hic31 and nacreous-layer matrix protein hic22 showed negative correlations with pearl weight, but the other seven nacreous-layer matrix proteins had significantly positive correlations with pearl weight. These results show the involvement of matrix proteins in pearl formation and in determination of quality traits.
引用
收藏
页码:240 / 249
页数:10
相关论文
共 46 条
  • [1] Mollusk shell formation: A source of new concepts for understanding biomineralization processes
    Addadi, L
    Joester, D
    Nudelman, F
    Weiner, S
    [J]. CHEMISTRY-A EUROPEAN JOURNAL, 2006, 12 (04) : 981 - 987
  • [2] Pearl formation: Persistence of the graft during the entire process of biomineralization
    Arnaud-Haond, S.
    Goyard, E.
    Vonau, V.
    Herbaut, C.
    Prou, J.
    Saulnier, D.
    [J]. MARINE BIOTECHNOLOGY, 2007, 9 (01) : 113 - 116
  • [3] Identification of housekeeping genes suitable for gene expression analysis in the pearl mussel, Hyriopsis cumingii, during biomineralization
    Bai, Zhiyi
    Lin, Jingyun
    Ma, Keyi
    Wang, Guiling
    Niu, Donghong
    Li, Jiale
    [J]. MOLECULAR GENETICS AND GENOMICS, 2014, 289 (04) : 717 - 725
  • [4] Cultured Pearl Surface Quality Profiling by the Shell Matrix Protein Gene Expression in the Biomineralised Pearl Sac Tissue of Pinctada margaritifera
    Blay, Carole
    Planes, Serge
    Ky, Chin-Long
    [J]. MARINE BIOTECHNOLOGY, 2018, 20 (04) : 490 - 501
  • [5] Correlations between cultured pearl size parameters and PIF-177 biomarker expression in Pinctada margaritifera families reared in two contrasting environments
    Blay, Carole
    Parrad, Sophie
    Cabral, Philippe
    Aiho, Vaite
    Ky, Chin-Long
    [J]. ESTUARINE COASTAL AND SHELF SCIENCE, 2016, 182 : 254 - 260
  • [6] Self-organisation of nacre in the shells of Pterioida (Bivalvia: Mollusca)
    Checa, AG
    Rodríguez-Navarro, AB
    [J]. BIOMATERIALS, 2005, 26 (09) : 1071 - 1079
  • [7] HcTyr and HcTyp-1 of Hyriopsis cumingii, novel tyrosinase and tyrosinase-related protein genes involved in nacre color formation
    Chen, Xiajun
    Liu, Xiaojun
    Bai, Zhiyi
    Zhao, Liting
    Li, Jiale
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2017, 204 : 1 - 8
  • [8] Investigation of cell proliferation and differentiation in the mantle of Pinctada fucata (Bivalve, Mollusca)
    Fang, Zi
    Feng, Qiaoli
    Chi, Yizhou
    Xie, Liping
    Zhang, Rongqing
    [J]. MARINE BIOLOGY, 2008, 153 (04) : 745 - 754
  • [9] Transcriptome-Guided Identification of Carbohydrate Active Enzymes (CAZy) from the Christmas Island Red Crab, Gecarcoidea natalis and a Vote for the Inclusion of Transcriptome-Derived Crustacean CAZys in Comparative Studies
    Gan, Han Ming
    Austin, Christopher
    Linton, Stuart
    [J]. MARINE BIOTECHNOLOGY, 2018, 20 (05) : 654 - 665
  • [10] Spatial analysis of biomineralization associated gene expression from the mantle organ of the pearl oyster Pinctada maxima
    Gardner, Luke D.
    Mills, David
    Wiegand, Aaron
    Leavesley, David
    Elizur, Abigail
    [J]. BMC GENOMICS, 2011, 12