An ornithine δ-aminotransferase gene OsOAT confers drought and oxidative stress tolerance in rice

Ornithine S-aminotransferase (delta-OAT) is a pyridoxa1-5'-phosphate-dependent enzyme that has been proposed to be involved in proline (Pro) and arginine (Arg) metabolism. However, the actual role of delta-OAT in abiotic responses in plants remains to be clarified. Here we characterized an ornithine delta-aminotransferase gene OsOAT that confers multi-stress tolerance in rice (Oryza sativa). We confirmed that OsOAT is a direct target of the stress-responsive NAC transcription factor SNAC2. OsOAT is responsive to multiple stresses and phytohormone treatments. Both ABA-dependent and ABA-independent pathways contributed to the drought-induced expression of OsOAT. Overexpression of the OsOAT gene in rice resulted in significantly enhanced drought and osmotic stress tolerance. Overexpression of OsOAT caused significantly increased delta-OAT activity and Pro accumulation under normal growth conditions. In addition, OsOAT-overexpressing plants showed significantly increased tolerance to oxidative stress. The glutathione (GSH) content and activity of reactive oxygen species (ROS)-scavenging enzymes, such as glutathione peroxidase, were also increased in OsOAT-overexpressing plants. We conclude that OsOAT is a target gene of SNAC2 and confers stress tolerance mainly through enhancing ROS-scavenging capacity and Pro pre-accumulation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.