In vivo assessment of cancerous tumors using boron doped diamond microelectrode

被引：56

作者：

Fierro, Stephane ^{[1
]}

Yoshikawa, Momoko ^{[2
]}

Nagano, Osamu ^{[2
]}

Yoshimi, Kenji ^{[3
]}

Saya, Hideyuki ^{[2
,4
]}

Einaga, Yasuaki ^{[1
,4
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Chem, Yokohama, Kanagawa 2238522, Japan

[2] Keio Univ, Inst Adv Med Res, Div Gene Regulat, Shinjuku Ku, Tokyo 1608582, Japan

[3] Juntendo Univ, Sch Med, Dept Neurophysiol, Bunkyo Ku, Tokyo 1138421, Japan

[4] JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

SCIENTIFIC REPORTS | 2012年 / 2卷

关键词：

LIQUID-CHROMATOGRAPHY; OXIDIZED GLUTATHIONE; ELECTRODES; SEPARATION; UV;

D O I：

10.1038/srep00901

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The in vitro and in vivo electrochemical detection of the reduced form of glutathione (L-gamma-glutamyl-L-cysteinyl-glycine, GSH) using boron doped diamond (BDD) microelectrode for potential application in the assessment of cancerous tumors is presented. Accurate calibration curve for the determination of GSH could be obtained by the in vitro electrochemical measurements. Additionally, it was shown that it was possible to separate the detection of GSH from the oxidized form of glutathione (GSSG) using chronoamperometry measurements. In vivo GSH detection measurements have been performed in human cancer cells inoculated in immunodeficient mice. These measurements have shown that the difference of GSH level between cancerous and normal tissues can be detected. Moreover, GSH detection measurements carried out before and after X-ray irradiation have proved that it is possible to assess in vivo the decrease in GSH concentration in the tumor after a specific treatment.

引用

页数：6

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