Enamel Formation Genes Influence Enamel Microhardness Before and After Cariogenic Challenge

被引：66

作者：

Shimizu, Takehiko ^{[2
]}

Ho, Bao ^{[1
]}

Deeley, Kathleen ^{[1
]}

Briseno-Ruiz, Jessica ^{[1
]}

Faraco, Italo M., Jr. ^{[1
]}

Schupack, Brett I. ^{[1
]}

Brancher, Joao A. ^{[4
]}

Pecharki, Giovana D. ^{[4
]}

Kuechler, Erika C. ^{[5
]}

Tannure, Patricia N. ^{[6
]}

Lips, Andrea ^{[7
]}

Vieira, Thays C. S. ^{[5
]}

Patir, Asli ^{[8
]}

Yildirim, Mine ^{[9
]}

Poletta, Fernando A. ^{[3
]}

Mereb, Juan C. ^{[10
]}

Resick, Judith M. ^{[1
]}

Brandon, Carla A. ^{[1
]}

Orioli, Ieda M. ^{[11
]}

Castilla, Eduardo E. ^{[3
,12
]}

Marazita, Mary L. ^{[1
,13
]}

Seymen, Figen ^{[9
]}

Costa, Marcelo C. ^{[7
]}

Granjeiro, Jose M. ^{[5
]}

Trevilatto, Paula C. ^{[4
]}

Vieira, Alexandre R. ^{[1
,14
]}

机构：

[1] Univ Pittsburgh, Dept Oral Biol, Pittsburgh, PA 15260 USA

[2] Nihon Univ Dent Matsudo, Dept Pediat Dent, Matsudo, Chiba, Japan

[3] Ctr Med Educ & Clin Res CEMIC, Latin Amer Collaborat Study Congenital Malformat, Buenos Aires, DF, Argentina

[4] Pontifical Catholic Univ Parana PUCPR, Ctr Hlth & Biol Sci, Curitiba, Parana, Brazil

[5] Univ Fed Fluminense, Inst Biol, Clin Res Unit, Niteroi, RJ, Brazil

[6] Veiga Almeida Univ, Discipline Pediat Dent, Rio De Janeiro, Brazil

[7] Univ Fed Rio de Janeiro, Dept Pediat Dent & Orthodont, Rio De Janeiro, Brazil

[8] Istanbul Medipol Univ, Dept Pedodont, Istanbul, Turkey

[9] Istanbul Univ, Fac Dent, Dept Pedodont, Istanbul, Turkey

[10] Hosp Area El Bolson, Latin Amer Collaborat Study Congenital Malformat, Rio Negro, Argentina

[11] Univ Fed Rio de Janeiro, Natl Inst Populat Med Genet INAGEMP CNPq, Latin Amer Collaborat Study Congenital Malformat, Dept Genet,Inst Biol,Ctr Hlth Sci, Rio De Janeiro, Brazil

[12] Fundacao Oswaldo Cruz, Latin Amer Collaborat Study Congenital Malformat, Natl Inst Populat Med Genet INAGEMP CNPq, Dept Genet, Rio De Janeiro, Brazil

[13] Univ Pittsburgh, Ctr Craniofacial & Dent Genet, Dept Human Genet & Clin & Translat Sci, Pittsburgh, PA USA

[14] Univ Pittsburgh, Sch Dent Med & Clin & Translat Sci, Dept Pediat Dent, Ctr Craniofacial & Dent Genet, Pittsburgh, PA USA

来源：

PLOS ONE | 2012年 / 7卷 / 09期

基金：

美国国家卫生研究院;

关键词：

HIGH CARIES EXPERIENCE; DENTAL-CARIES; ASSOCIATION; AMELOGENIN; FISSURE; PIT;

D O I：

10.1371/journal.pone.0045022

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

There is evidence for a genetic component in caries susceptibility, and studies in humans have suggested that variation in enamel formation genes may contribute to caries. For the present study, we used DNA samples collected from 1,831 individuals from various population data sets. Single nucleotide polymorphism markers were genotyped in selected genes (ameloblastin, amelogenin, enamelin, tuftelin, and tuftelin interacting protein 11) that influence enamel formation. Allele and genotype frequencies were compared between groups with distinct caries experience. Associations with caries experience can be detected but they are not necessarily replicated in all population groups and the most expressive results was for a marker in AMELX (p = 0.0007). To help interpret these results, we evaluated if enamel microhardness changes under simulated cariogenic challenges are associated with genetic variations in these same genes. After creating an artificial caries lesion, associations could be seen between genetic variation in TUFT1 (p = 0.006) and TUIP11 (p = 0.0006) with enamel microhardness. Our results suggest that the influence of genetic variation of enamel formation genes may influence the dynamic interactions between the enamel surface and the oral cavity.

引用

页数：9

共 21 条

[1] GOLD - Graphical Overview of Linkage Disequilibrium [J].