Enzyme-linked immunosorbent assay to detect urinary antibody against recombinant rKRP42 antigen made from Leishmania donovani for the diagnosis of visceral leishmaniasis

被引:21
作者
Islam, Mohammad Zahidul [1 ]
Itoh, Makoto
Takagi, Hidekazu
Islam, Anwar U.
Ekram, A. R. M. Saifuddin
Rahman, Ajijur
Takesue, Atsuhide
Hashiguchi, Yoshihisa
Kimura, Eisaku
机构
[1] Aichi Med Univ, Sch Med, Dept Parasitol, Nagakute, Aichi 4801195, Japan
基金
日本学术振兴会;
关键词
D O I
10.4269/ajtmh.2008.79.599
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
We recently reported the production of the recombinant kinesin-related protein of Leishmania donovani with a molecular weight of 42 kd (rKRP42) and the value of the antigen in serum-based ELISA for the diagnosis of visceral leishmaniasis (VL). In this study, the rKRP42 antigen was validated with ELISA using urine samples (rKRP42 urine ELISA). The urine-based ELISA showed 94% sensitivity (108 positives among 115 VL samples) and 99.6% specificity (239 negatives among 240 non-VL samples). The sensitivity and specificity are almost similar to our previous results by ELISA with acetone-treated L. donovani promastigote antigen and direct agglutination test, both methods being done by use of urine samples. A comparison of the rKRP42 urine ELISA with the commercially available urinary antigen detection kit (KAtex) using 108 VL samples showed much higher sensitivity of the ELISA (96.3%) than KAtex (55.6%). The use of the rKRP42 antigen with urine samples will facilitate epidemiologic studies.
引用
收藏
页码:599 / 604
页数:6
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