Identification of Baiying (Herba Solani Lyrati) commodity and its toxic substitute Xungufeng (Herba Aristolochiae Mollissimae) using DNA barcoding and chemical profiling techniques

被引:26
作者
Li, Ming [2 ]
Au, Ka-Yee [1 ]
Lam, Hilary [1 ]
Cheng, Ling [2 ]
Jiang, Ren-Wang [3 ]
But, Paul Pui-Hay [1 ]
Shaw, Pang-Chui [1 ,2 ]
机构
[1] Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
[2] Chinese Univ Hong Kong, Inst Chinese Med, State Key Lab Phytochem & Plant Resources W China, Shatin, Hong Kong, Peoples R China
[3] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constituents, Guangzhou, Guangdong, Peoples R China
关键词
Aristolochia mollissima; Aristolochic acids; Authentication; DNA barcoding; Solanum lyratum; ACID; AUTHENTICATION; AMPLIFICATION; NOMENCLATURE; MISUSE; WEB;
D O I
10.1016/j.foodchem.2012.06.049
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Baiying derived from Solanum lyratum Hance is a commonly consumed natural product for ethnomedical treatment of cancer. One of the substitutes present in the market is a carcinogenic aristolochic acids-containing herb Xungufeng derived from Aristolochia mollissima Thunb. The purpose of this study is to establish DNA barcodes, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and cytotoxicity assay to differentiate Baiying from Xungufeng. A total of 30 DNA sequences from five DNA barcodes (ITS, matK, rbcL, trnH-psbA and trnL-trnF) were generated to differentiate S. lyratum from A. mollissima and authenticate ten samples of Baiying and Xungufeng commodities. Using aristolochic acids as standard markers, TLC and HPLC analyses also successfully authenticated these commodities. In vitro cytotoxicity assay using HEK-293 and Vero cells demonstrated that Xungufeng was significantly more toxic than Baiying. This is the first study applying an integrated molecular, chemical and biological approach to differentiate traditional Chinese medicine from Aristolochia adulterant. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1653 / 1658
页数:6
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