A novel method for the immobilization of glucoamylase to produce glucose from maltodextrin

被引：33

作者：

Tanriseven, A ^{[1
]}

Uludag, YB ^{[1
]}

Dogan, S ^{[1
]}

机构：

[1] Gebze Inst Technol, Dept Biochem, Gebze, Kocaeli, Turkey

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 03期

关键词：

alginate; glucoamylase; glucose; immobilization; maltodextrin; starch;

D O I：

10.1016/S0141-0229(02)00004-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immobilized glucoamylase from Aspergillus niger was used to produce glucose from maltodextrin, The enzyme was immobilized using a newly developed combined method of adsorption of the enzyme to gelatinized corn starch and Subsequent alginate fiber entrapment. The immobilization resulted in 22% relative activity and has been active for 21 days without decrease in activity. The optimum conditions were not affected by immobilization and the optimum pH and temperature for free and immobilized enzyme were 4.3 and 60degreesC, respectively, The kinetic parameters were also determined for the hydrolysis of maltodextrin by free and immobilized glucoamylase. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：406 / 409

页数：4

共 12 条

[1] Characterization of glucoamylase immobilized on magnetic poly(styrene) particles [J].

Bahar, T ;

Çelebi, SS .

ENZYME AND MICROBIAL TECHNOLOGY, 1998, 23 (05) :301-304

[2] COVALENT STABILIZATION OF ALGINATE GEL FOR THE ENTRAPMENT OF LIVING WHOLE CELLS [J].

BIRNBAUM, S ;

PENDLETON, R ;

LARSSON, PO ;

MOSBACH, K .

BIOTECHNOLOGY LETTERS, 1981, 3 (08) :393-400

[3]

CHITRA N, 1989, PROCESS BIOCHEM, V12, P208

[4] CARBODIIMIDE COUPLING OF BETA-GALACTOSIDASE FROM ASPERGILLUS-ORYZAE TO ALGINATE [J].

DOMINGUEZ, E ;

NILSSON, M ;

HAHNHAGERDAL, B .

ENZYME AND MICROBIAL TECHNOLOGY, 1988, 10 (10) :606-610

[5]

DUNCOMBE GR, 1984, Patent No. 4430348

[6]

Grosz J., 1989, Journal of Planar Chromatography, V2, P420

[7] EFFECT OF SUBSTRATE PRESOAKING TREATMENT OF SUPPORT MATERIALS ON THE ACTIVITY OF IMMOBILIZED GLUCOAMYLASE [J].

MUKATAKA, S ;

NEGISHI, S ;

SATO, S ;

TAKAHASHI, J .

ENZYME AND MICROBIAL TECHNOLOGY, 1993, 15 (03) :229-233

[8] Preparation and properties of immobilized amyloglucosidase on nonporous PS/PNaSS microspheres [J].

Oh, JT ;

Kim, JH .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (06) :356-361

[9] UNCONVENTIONAL IMMOBILIZATION OF DEXTRANSUCRASE WITH ALGINATE [J].

REISCHWITZ, A ;

REH, KD ;

BUCHHOLZ, K .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (05) :457-461

[10] Steady-state kinetic and calorimetric studies on the binding of Aspergillus niger glucoamylase with gluconolactone, 1-deoxynojirimycin, and beta-cyclodextrin [J].

Tanaka, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (12) :2055-2058

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