Sol-Gel-Derived Silver-Nanoparticle-Embedded Thin Film for Mass Spectrometry-Based Biosensing

被引：35

作者：

Gamez, Roberto C. ^{[1
]}

Castellana, Edward T. ^{[1
]}

Russell, David H. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, Lab Biol Mass Spectrometry, College Stn, TX 77843 USA

来源：

LANGMUIR | 2013年 / 29卷 / 21期

关键词：

LASER-DESORPTION IONIZATION; RAY PHOTOELECTRON-SPECTROSCOPY; SURFACE CHARACTERIZATION; 2,5-DIHYDROXYBENZOIC ACID; THERMAL-DECOMPOSITION; POROUS SILICON; DESORPTION/IONIZATION; AG; MATRIX; BIOMOLECULES;

D O I：

10.1021/la4008526

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A porous silver-nanoparticle (AgNP)-embedded thin film biosensor was produced by the sol-gel method. The thin films were used as matrix-free laser desorption ionization mass spectrometry (LDI-MS) biosensors applicable to several chemical classes. In these experiments, UV laser irradiation (337 nm) of the AgNP facilitates desorption and ionization of a number of peptides, triglycerides, and phospholipids. Preferential ionization of sterols from vesicles composed of olefinic phosphosphatidylcholines is also demonstrated, offering the possibility for a simplified approach for sterol analysis from complex mixtures. The composition of the nanoparticles was confirmed by X-ray photoelectron spectroscopy (XPS) and UV-vis spectroscopy. XPS data revealed a binding energy of 368.2 eV, consistent with the previous assignment of the binding energy for the Ag 3d(5/2) peak from Ag-0 at 368.1 +/- 0.1 eV. The surface morphology of the thin films was studied by field-emission scanning electron microscopy (FE-SEM) and revealed the presence of nanoparticles and the porous nature of the biosensor.

引用

页码：6502 / 6507

页数：6

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