Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions

被引：26

作者：

Chen, J ^{[1
]}

Zhang, JL ^{[1
]}

Han, BX ^{[1
]}

Li, ZH ^{[1
]}

Li, JC ^{[1
]}

Feng, XY ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2006年 / 48卷 / 01期

基金：

中国国家自然科学基金;

关键词：

CLEAs; enzyme; reverse micelles; CO2;

D O I：

10.1016/j.colsurfb.2006.01.010

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new method to prepare the cross-linked enzyme aggregates (CLEAs) was developed. Through cross-linking the enzyme (Trypsin) aggregates, which was precipitated from the CO2-expanded reverse micellar solutions, dendritic CLEAs were obtained. The sizes of the CLEAs prepared by this new method were nanometer order of magnitudes and could be tuned by changing the water-to-surfactant ratio (w(0)) and the concentration of enzyme in the reverse micellar solution. The diameter of CLEAs increased with increasing w(0) value of reverse micelles and the concentration of Trypsin. The activity of CLEAs obtained by this method is improved in contrast to those obtained by the conventional method. This method has some advantages in applications and can be easily applied to the synthesis of other cross-linked enzyme aggregates. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：72 / 76

页数：5

共 40 条

[1] Cross-linked aggregates of penicillin acylase:: robust catalysts for the synthesis of β-lactam antibiotics [J].

Cao, L ;

van Langen, LM ;

van Rantwijk, F ;

Sheldon, RA .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 11 (4-6) :665-670

[2] Immobilised enzymes: carrier-bound or carrier-free? [J].

Cao, LQ ;

van Langen, L ;

Sheldon, RA .

CURRENT OPINION IN BIOTECHNOLOGY, 2003, 14 (04) :387-394

[3] Cross-linked enzyme aggregates: A simple and effective method for the immobilization of penicillin acylase [J].

Cao, LQ ;

van Rantwijk, F ;

Sheldon, RA .

ORGANIC LETTERS, 2000, 2 (10) :1361-1364

[4] Supercritical CO2-based production of fullerene nanoparticles [J].

Chattopadhyay, P ;

Gupta, RB .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2000, 39 (07) :2281-2289

[5] Investigation on the precipitation, microenvironment and recovery of protein in CO2-expanded reverse micellar solution [J].

Chen, J ;

Zhang, JL ;

Liu, DX ;

Liu, ZM ;

Han, BX ;

Yang, GY .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2004, 33 (01) :33-37

[6] MICROCELLULAR MICROSPHERES AND MICROBALLOONS BY PRECIPITATION WITH A VAPOR-LIQUID COMPRESSED FLUID ANTISOLVENT [J].

DIXON, DJ ;

LUNABARCENAS, G ;

JOHNSTON, KP .

POLYMER, 1994, 35 (18) :3998-4005

[7]

Drauz K., 1995, ENZYME CATALYSIS ORG

[8] Supercritical CO2 extraction of oil and residual proteins from Atlantic mackerel (Scomber scombrus) as affected by moisture content [J].

Dunford, NT ;

Temelli, F ;

LeBlanc, E .

JOURNAL OF FOOD SCIENCE, 1997, 62 (02) :289-294

[9]

FABER K, 1997, BIOTRANSFORMATION OR

[10] Multiphase multicomponent equilibria for mixtures containing polymers by the perturbation theory [J].

Favari, F ;

Bertucco, A ;

Elvassore, N ;

Fermeglia, M .

CHEMICAL ENGINEERING SCIENCE, 2000, 55 (13) :2379-2392

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