The conserved Ala37 in the ERF/AP2 domain is essential for, binding with the DRE element and the GCC box

被引：93
作者：
Liu, Y
Zhao, TJ
Liu, JM
Liu, WQ
Liu, Q
Yan, YB [1 ]
Zhou, HM
机构：
[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
[3] Tsing Hua Univ, Minist Educ, Prot Sci Lab, Beijing 100084, Peoples R China
[4] Tsing Hua Univ, Yangtze Delta Reg Inst, Jiaxing 314050, Peoples R China
来源：
FEBS LETTERS | 2006年 / 580卷 / 05期
基金：
中国国家自然科学基金;
关键词：
BnDREBIII; dehydration-responsive element; GCC box; ERF/AP2; domain; Brassica napus;
D O I：
10.1016/j.febslet.2006.01.048
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：1303 / 1308
页数：6
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