Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell

被引：196

作者：

Huang, Yee ^{[1
]}

Jiang, Chunmao ^{[2
]}

Hu, Yuanliang ^{[1
]}

Zhao, Xiaojuan ^{[1
]}

Shi, Cheng ^{[1
]}

Yu, Yun ^{[1
]}

Liu, Cui ^{[1
]}

Tao, Yang ^{[1
]}

Pan, Huarong ^{[1
]}

Feng, Yibo ^{[1
]}

Liu, Jiaguo ^{[1
]}

Wu, Yi ^{[1
]}

Wang, Deyun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Anim Husb & Vet Coll, Taizhou 225300, Jiangsu, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2013年 / 96卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Rehmannia glutinosa polysaccharide; Lymphocyte proliferation; Dendritic cell; Immunoenhancement; IL-2; IFN-gamma; ANTIGEN PRESENTATION; T-CELLS; RADIX; IMMUNOPATHOLOGY; EXPRESSION; RESPONSES; CATALPOL;

D O I：

10.1016/j.carbpol.2013.04.018

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48 h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MU method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and 1FN-gamma production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：516 / 521

页数：6

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