Secreted Factors from Keloid Keratinocytes Modulate Collagen Deposition by Fibroblasts from Normal and Fibrotic Tissue: A Pilot Study

被引：7

作者：

Alghamdi, Mansour A. ^{[1
,2
]}

AL-Eitan, Laith N. ^{[3
,4
]}

Stevenson, Andrew ^{[5
]}

Chaudhari, Nutan ^{[5
]}

Hortin, Nicole ^{[5
]}

Wallace, Hilary J. ^{[5
,6
]}

Danielsen, Patricia L. ^{[7
,8
]}

Manzur, Mitali ^{[9
]}

Wood, Fiona M. ^{[5
,10
,11
,12
]}

Fear, Mark W. ^{[5
]}

机构：

[1] King Khalid Univ, Dept Anat, Coll Med, Abha 61421, Saudi Arabia

[2] King Khalid Univ, Coll Med, Genom & Personalized Med Unit, Abha 61421, Saudi Arabia

[3] Jordan Univ Sci & Technol, Dept Appl Biol Sci, Irbid 22110, Jordan

[4] Jordan Univ Sci & Technol, Dept Biotechnol & Genet Engn, Irbid 22110, Jordan

[5] Univ Western Australia, Burn Injury Res Unit, Sch Biomed Sci, Fac Hlth & Med Sci, 35 Stirling Highway, Crawley 6009, Australia

[6] Univ Notre Dame Australia, Sch Med, Fremantle, WA 6959, Australia

[7] Copenhagen Univ Hosp, Dept Dermatol, DK-2400 Copenhagen NV, Denmark

[8] Copenhagen Univ Hosp, Copenhagen Wound Healing Ctr, DK-2400 Copenhagen NV, Denmark

[9] Univ Western Australia, Telethon Kids Inst, Perth Childrens Hosp, Nedlands, WA 6009, Australia

[10] Perth Childrens Hosp, Burns Serv Western Australia, Perth, WA 6009, Australia

[11] Fiona Stanley Hosp, Dept Hlth, Perth, WA 6009, Australia

[12] Fiona Stanley Hosp, Fiona Wood Fdn, Perth, WA 6150, Australia

来源：

BIOMEDICINES | 2020年 / 8卷 / 07期

关键词：

keloids; keratinocytes; collagen type I; wound healing; fibroblasts; coculture techniques; EPITHELIAL-MESENCHYMAL INTERACTIONS; EXPRESSION; PATHOGENESIS; GROWTH; PROLIFERATION; CULTIVATION; COCULTURE; DENSITY; SCARS; SKIN;

D O I：

10.3390/biomedicines8070200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interactions between keratinocytes and fibroblasts in the skin layers are crucial in normal tissue development, wound healing, and scarring. This study has investigated the role of keloid keratinocytes in regulating collagen production by primary fibroblasts in vitro. Keloid cells were obtained from removed patients' tissue whereas normal skin cells were discarded tissue obtained from elective surgery procedures. Fibroblasts and keratinocytes were isolated, cultured, and a transwell co-culture system were used to investigate the effect of keratinocytes on collagen production using a 'scar-in-a-jar' model. Keloid fibroblasts produced significantly more collagen than normal skin fibroblasts in monoculture at the RNA, secreted protein, and stable fibrillar protein level. When keloid keratinocytes were added to normal skin fibroblasts, expression of collagen was significantly upregulated in most samples, but when added to keloid fibroblasts, collagen I production was significantly reduced. Interestingly, keloid keratinocytes appear to decrease collagen production by keloid fibroblasts. This suggests that signaling in both keratinocytes and fibroblasts is disrupted in keloid pathology.

引用

页数：14

共 33 条

[1] Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells [J].