Prediction and identification of an acid-inducible promoter from Lactococcus lactis ssp cremoris MG1363

被引：1
|
作者：
Yu, Hongxian [1 ]
Xu, Wentao [1 ,2 ]
Luo, Yunbo [1 ]
He, Jing [1 ]
Tian, Hongtao [3 ]
Shi, Hui [1 ]
Mei, Xiaohong [1 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Lab Food Safety & Mol Biol, Beijing 100083, Peoples R China
[2] Minist Agr, Supervis Inspect & Testing Ctr Genet Modified Org, Beijing 100083, Peoples R China
[3] Agr Univ Hebei, Lab Food Biotechnol, Baoding 071001, Hebei Province, Peoples R China
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2015年 / 24卷 / 05期
关键词：
Lactococcus lactis; acid-inducible promoter; green fluorescent protein; HETEROLOGOUS PROTEIN-PRODUCTION; ESCHERICHIA-COLI; LACTOBACILLUS-PLANTARUM; BACILLUS-SUBTILIS; GENE-EXPRESSION; SEQUENCES; REGIONS; CLONING; GENOME; LEVEL;
D O I：
10.1007/s10068-015-0227-4
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Using a promoter online prediction system, the gene sequence of GroEL protein from Lactococcus lactis ssp. cremoris MG1363 was analysed and a putative promoter, designated as gro, was further studied. Under acidic conditions, gro showed preliminary promoter function by driving the expression of lacZ reporter gene in Escherichia coli DH5 alpha. In addition, gro drove the gene expression of GroEL and green fluorescent protein. In this paper, an acid-inducible promoter was successfully identified. This promoter has a wide prospect in the field of biotechnology.
引用
收藏
页码：1749 / 1753
页数：5
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