Label-free mass spectrometry-based proteomics for biomarker discovery and validation

被引：1

作者：

Pham, Thang V. ^{[1
]}

Piersma, Sander R. ^{[1
]}

Oudgenoeg, Gideon ^{[1
]}

Jimenez, Connie R. ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Med Ctr, Dept Med Oncol, Oncoprote Lab CCA 160, NL-1081 NV Amsterdam, Netherlands

来源：

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS | 2012年 / 12卷 / 04期

关键词：

biomarker discovery; label-free proteomics; selected reaction monitoring; spectral counting; LASER CAPTURE MICRODISSECTION; COMPLEX PROTEIN MIXTURES; SPECTRAL COUNT DATA; QUANTITATIVE PROTEOMICS; CELL-CULTURE; AMINO-ACIDS; RELATIVE QUANTIFICATION; ABSOLUTE QUANTIFICATION; GEL-ELECTROPHORESIS; COLON-CANCER;

D O I：

10.1586/ERM.12.31

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

For most diseases, better biomarkers are urgently needed to enable (early) detection, diagnosis, prognosis, stratification for therapy and response monitoring. Proteomics delineates gene products that carry out the majority of cellular functions, and thereby may not only yield insight into altered signaling pathways in disease, but also yield novel biomarkers. In recent years, great progress has been made in mass spectrometry-based analysis of clinical tissues and biofluids, with identification and quantification of thousands of proteins now becoming increasingly routine. However, biomarker validation and clinical translation has turned out to be challenging. In this review, we summarize current mass spectrometry-based proteomics strategies for biomarker discovery and verification using selected reaction monitoring, with a focus on progress and recent applications in clinical material using label-free approaches.

引用

页码：343 / 359

页数：17

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