Caffeic Acid Detection Using an Inhibition-Based Lipoxygenase Sensor

被引：12
作者：
Demirkol, Dilek Odaci [1 ]
Gulsunoglu, Baran [1 ]
Ozdemir, Caglar [1 ]
Dincer, Ayse [1 ]
Zihnioglu, Figen [1 ]
Timur, Suna [1 ]
机构：
[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
来源：
FOOD ANALYTICAL METHODS | 2012年 / 5卷 / 02期
关键词：
Biosensor; Lipoxygenase; Caffeic acid; Carbon paste electrode; PERFORMANCE LIQUID-CHROMATOGRAPHY; PHENOLIC-ACIDS; CAPILLARY-ELECTROPHORESIS; ELECTROCHEMICAL DETECTION; AMPEROMETRIC DETECTION; ANTIOXIDANT ACTIVITY; FRUIT JUICES; ELECTRODE; ENZYME; POLYPHENOLS;
D O I：
10.1007/s12161-011-9226-0
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
An inhibition based biosensing system was developed for the caffeic acid as lipoxygenase (LOX) inhibitor. LOX was immobilized in carbon paste electrode and the amperometric detection of hydroperoxy linoleic acid due to the enzymatic reaction using linoleic acid as a substrate was monitored at +0.9 V versus Ag/AgCl. The decrease in biosensor response in the presence of caffeic acid was found to be correlated with the inhibitor concentration. Diode array detector and LOX biosensor was used as an electrochemical detector for the analysis of this compound. All data were given as a comparison of two systems.
引用
收藏
页码：244 / 249
页数：6
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