Biocatalysts Characters of Novel Bacteria from Crude Oil-Contaminated River

The potential of several biocatalysts produced by bacteria BF2 isolated from crude oil-contaminated river were highly associated to the bioremediation mechanism of that pollutant. The molecular identification and production of key enzymes of the strain such as catalase, lipase, and oxidoreductase, were very important to investigate. Based on 16S rDNA sequence which subjected to BLASTN (NCBI) database queries, the strain was identified of Stenotrophomonas pavanii. This strain was showed the optimal growth in Bushnell Hass Mineral salts (BHMS) medium containing 1 % of crude oil. This strain could also degrade that crude oil in medium, achieving a 62.5 % reduction of total petroleum hydrocarbons (TPH) after 9 days incubation. Therefore, the crude extracts of enzymes obtained at that time were measured of their specific enzyme activities by producing several biocatalysts such as catalase (25.3 U/mg protein), lipase (48 U/mg protein), and oxidoreductase (39.2 U/mg protein). These results indicated that the strain effective in removing hydrocarbon in the crude oil pollutant by producing highly enzymes of the catalase, lipase, and oxidoreductase.