Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression

被引：157
作者：
Gui, Dingkun [1 ]
Huang, Jianhua [2 ]
Guo, Yongping [1 ]
Chen, Jianguo [3 ]
Chen, Yifang [3 ]
Xiao, Wenzhen [1 ]
Liu, Xusheng [4 ]
Wang, Niansong [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Dept Nephrol & Rheumatol, Peoples Hosp 6, Shanghai 200030, Peoples R China
[2] Fudan Univ, Inst Integrated Chinese & Western Med, Huashan Hosp, Shanghai 200433, Peoples R China
[3] Zhejiang Hosp, Dept Nephrol, Hangzhou, Zhejiang, Peoples R China
[4] Guangdong Prov Hosp Tradit Chinese Med, Nephrol Ctr, Guangzhou, Guangdong, Peoples R China
来源：
CYTOKINE | 2013年 / 61卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Astragaloside IV; Diabetic nephropathy; Inflammation; NF-kappa B; MONOCYTE CHEMOATTRACTANT PROTEIN-1; CONVERTING ENZYME-INHIBITION; ADHESION MOLECULE-1 ICAM-1; NECROSIS-FACTOR-ALPHA; UP-REGULATION; SODIUM RETENTION; MESANGIAL CELLS; NEPHROPATHY; ACTIVATION; PATHWAY;
D O I：
10.1016/j.cyto.2013.01.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Accumulating evidence suggests that inflammatory processes are involved in the development of diabetic nephropathy (DN). However, there are no effective interventions for inflammation in the diabetic kidneys. Here, we tested the hypothesis that Astragaloside IV(AS-IV), a novel saponin purified from Astragalus membranaceus (Fisch) Bge, ameliorates DN in streptozotocin (STZ)-induced diabetic rats through anti-inflammatory mechanisms. Diabetes was induced with STZ (65 mg/kg) by intraperitoneal injection in rats. Two weeks after STZ injection, rats were divided into three groups (n = 8/each group), namely, diabetic rats, diabetic rats treated with AS-IV at 5 and 10 mg kg(-1) d(-1), p.o., for 8 weeks. The normal rats were chosen as nondiabetic control group (n = 8). The rats were sacrificed 10 weeks after induction of diabetes. AS-IV ameliorated albuminuria, renal histopathology and podocyte foot process effacement in diabetic rats. Renal NF-kappa B activity, as wells as protein and mRNA expression were increased in diabetic kidneys, accompanied by an increase in mRNA expression and protein content of TNF-alpha, MCP-1 and ICAM-1 in kidney tissues. The alpha(1)-chain type IV collagen mRNA was elevated in the kidneys of diabetic rats. All of these abnormalities were partially restored by AS-IV. AS-IV also decreased the serum levels of TNF-alpha, MCP-1 and ICAM-1 in diabetic rats. These findings suggest that AS-IV, a novel anti-inflammatory agent, attenuated DN in rats through inhibiting NE-kappa B mediated inflammatory genes expression. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：970 / 977
页数：8
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