Acrylic-Acid-Functionalized PolyHIPE Scaffolds for Use in 3D Cell Culture

被引：58
作者：
Hayward, Adam S. [1 ]
Sano, Naoko [2 ]
Przyborski, Stefan A. [1 ]
Cameron, Neil R. [3 ]
机构：
[1] Univ Durham, Sch Biol & Biomed Sci, Durham DH1 3LE, England
[2] Newcastle Univ, Sch Mech & Syst Engn, NEXUS, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[3] Univ Durham, Dept Chem, Durham DH1 3LE, England
来源：
MACROMOLECULAR RAPID COMMUNICATIONS | 2013年 / 34卷 / 23-24期
基金：
英国工程与自然科学研究理事会;
关键词：
acrylic acid; polyHIPE; polystyrene; scaffold; 3D cell culture; TEMPLATED POROUS POLYMERS; INTERNAL PHASE EMULSIONS; MACROPOROUS POLYHIPE; OSTEOBLAST GROWTH; IN-VITRO; TISSUE; MORPHOLOGY; FOAMS; POLYMERIZATION; HEPATOCYTES;
D O I：
10.1002/marc.201300709
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89%, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5% carboxylic acid functionality, providing a useful substrate for subsequent surface modifications and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.
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收藏
页码：1844 / 1849
页数：6
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