Spectroscopic study one thiosemicarbazone derivative with ctDNA using ethidium bromide as a fluorescence probe

被引：31
作者：
Geng, Shaoguang [1 ]
Wu, Qing [2 ]
Shi, Lei [1 ]
Cui, Fengling [1 ]
机构：
[1] Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China
[2] Henan Normal Univ, Sch Environm, Xinxiang 453007, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 60卷
基金：
中国国家自然科学基金;
关键词：
(E)-2-((1,4-dihydroxy-9,10-anthraquinone-2-yl) methylene)-N-(4-fluorophenyl); hydrazinecarbothioamide (DAFPT); Calf thymus DNA (ctDNA); Ethidium bromide (EB); Fluorescence spectroscopy; Molecular docking; NEUTRAL RED-DYE; ACRIDINE-ORANGE; DNA INTERACTION; SYNCHRONOUS FLUORESCENCE; INTERACTION MECHANISM; COMPLEX; BINDING; FLAVONOIDS; BLUE;
D O I：
10.1016/j.ijbiomac.2013.06.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, a thiosemicarbazone derivative (E)-2-((1,4-dihydroxy-9,10-anthraquinone-2yl)methylene)-N-(4-fluorophenyl)hydrazinecarbothioamide (DAFPT) was synthesized, and the interaction of DAFPT with calf thymus DNA (ctDNA) was explored using ethidium bromide (EB) as a fluorescence probe. The binding mode between DAFPT and ctDNA was investigated by UV absorption spectroscopy, fluorescence spectroscopy and molecular docking. The fluorescence quenching mechanism of EB-ctDNA by DAFPT might be a combined quenching type. Thermodynamic parameters showed that the reaction was spontaneous. According to ionic strength, fluorescence polarization and melting temperature (Tm) curve results, DAFPT-ctDNA interaction was groove binding. The molecular modeling results indicated that DAFPT could slide into the A-T rich region of ctDNA. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：288 / 294
页数：7
相关论文
共 37 条
[1]   Spectroscopic and electrochemical properties of di-2-pyridyl ketone thiosemicarbazone [dpktsc] in non-aqueous media [J].
Bakir, Mohammed ;
Brown, Ordel .
JOURNAL OF MOLECULAR STRUCTURE, 2011, 1006 (1-3) :402-408
[2]   Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method [J].
Bi, Shuyun ;
Zhang, Hanqi ;
Qiao, Chunyu ;
Sun, Ying ;
Liu, Chunming .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2008, 69 (01) :123-129
[3]   Study of interactions of flavonoids with DNA using acridine orange as a fluorescence probe [J].
Bi, Shuyun ;
Qiao, Chunyu ;
Song, Daqian ;
Tian, Yuan ;
Gao, Dejiang ;
Sun, Ying ;
Zhang, Hanqi .
SENSORS AND ACTUATORS B-CHEMICAL, 2006, 119 (01) :199-208
[4]   Interaction of a novel red-region fluorescent probe, Nile Blue, with DNA and its application to nucleic acids assay [J].
Chen, QG ;
Li, DH ;
Zhao, Y ;
Yang, HH ;
Zhu, QZ ;
Xu, JG .
ANALYST, 1999, 124 (06) :901-906
[5]   Interaction of 3′-azido-3′-deamino daunorubicin with DNA: Multispectroscopic and molecular modeling [J].
Cui, Fengling ;
Hui, Guangquan ;
Jiang, Xiaoying ;
Zhang, Guisheng .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 50 (04) :1121-1126
[6]   A review on DNA interaction with synthetic phenolic food additives [J].
Dolatabadi, Jafar Ezzati Nazhad ;
Kashanian, Soheila .
FOOD RESEARCH INTERNATIONAL, 2010, 43 (05) :1223-1230
[7]   Synthesis and DNA interaction of ethylenediamine platinum(II) complexes linked to DNA intercalants [J].
Duskova, Katerina ;
Sierra, Sara ;
Fernandez, Maria-Jose ;
Gude, Lourdes ;
Lorente, Antonio .
BIOORGANIC & MEDICINAL CHEMISTRY, 2012, 20 (24) :7112-7118
[8]   Molecular interaction of ctDNA and HSA with sulfadiazine sodium by multispectroscopic methods and molecular modeling [J].
Geng, Shaoguang ;
Liu, Guosheng ;
Li, Wei ;
Cui, Fengling .
LUMINESCENCE, 2013, 28 (05) :785-792
[9]   Spectroscopic and molecular modeling study on the interaction of ctDNA with 3′-deoxy-3′-azido doxorubicin [J].
Geng, Shaoguang ;
Cui, Yanrui ;
Liu, Qingfeng ;
Cui, Fengling ;
Zhang, Guisheng ;
Chi, Yanwei ;
Peng, Hao .
JOURNAL OF LUMINESCENCE, 2013, 141 :144-149
[10]   Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE) [J].
Gherghi, IC ;
Girousi, ST ;
Voulgaropoulos, AN ;
Tzimou-Tsitouridou, R .
TALANTA, 2003, 61 (02) :103-112
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