DDN-AS1-miR-15a/16-TCF3 feedback loop regulates tumor progression in cervical cancer

被引：22

作者：

Liu, Zhihui ^{[1
]}

Wu, Meiqin ^{[1
]}

Shi, Huifeng ^{[2
]}

Huang, Chong ^{[1
]}

Luo, Sukun ^{[3
]}

Song, Xiaojie ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Dept Gynecol, 213 Qiuchang Rd, Wuhan 430016, Hubei, Peoples R China

[2] Second Peoples Hosp Nanhai Dist, Dept Gynecol, Foshan, Guangdong, Peoples R China

[3] Huazhong Univ Sci & Technol, Cent Lab, Wuhan Childrens Hosp, Tongji Med Coll, Wuhan, Hubei, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 06期

关键词：

cervical cancer; lncRNA DDN and PRKAG1 antisense RNA 1; migration; miR-15a; 16; transcription factor 3; LONG NONCODING RNA; COMPETING ENDOGENOUS RNA; CARCINOMA; METASTASIS; PROLIFERATION; TRANSCRIPTION; EXPRESSION; GROWTH;

D O I：

10.1002/jcb.28307

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cervical cancer (CC) is known as one of the most common gynecological tumors. Long noncoding RNAs (lncRNAs) are a group of regulators that have been widely reported in human malignant tumors including CC. On the basis of the data of The Cancer Genome Atlas, lncRNA DDN and PRKAG1 antisense RNA 1 (DDN-AS1) that is overexpressed in CC tissues predicted poor prognosis for patients with CC. Moreover, quantitative reverse transcription PCR analysis further identified the upregulation of DDN-AS1 in CC tissues and cell lines. Loss-of-function assays revealed that knockdown of DDN-AS1 suppressed CC progression by efficiently inhibiting cell proliferation, migration, and invasion. Mechanism investigations revealed that DDN-AS1 was upregulated by its upstream transcription activatortranscription factor 3 (TCF3). Moreover, DDN-AS1 increased the expression ofTCF3 by competitively binding miR-15a and miR-16. In conclusion, DDN-AS1-miR-15a/16-TCF3 feedback loop contributes to cell proliferation, migration, and invasion in CC.

引用

页码：10228 / 10238

页数：11

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