Single Cell Time-lapse Imaging of Focus Formation by the DNA Damage-Response Protein 53BP1 after UVC Irradiation of Human Pancreatic Cancer Cells

被引：0
作者：
Miwa, Shinji [1 ,2 ,3 ]
Tome, Yasunori [1 ,2 ,4 ]
Yano, Shuya [1 ,2 ]
Hiroshima, Yukihiko [1 ,2 ]
Uehara, Fuminari [1 ,2 ,4 ]
Mii, Sumiyuki [1 ,2 ]
Kimura, Hiroaki [3 ]
Hayashi, Katsuhiro [3 ]
Tsuchiya, Hiroyuki [3 ]
Bouvet, Michael [2 ]
Efimova, Elena V. [5 ]
Hoffman, Robert M. [1 ,2 ]
机构：
[1] AntiCanc Inc, San Diego, CA 92111 USA
[2] Univ Calif San Diego, Dept Surg, San Diego, CA 92103 USA
[3] Kanazawa Univ, Grad Sch Med Sci, Dept Orthopaed Surg, Kanazawa, Ishikawa, Japan
[4] Univ Ryukyus, Grad Sch Med, Dept Orthoped Surg, Okinawa, Japan
[5] Univ Chicago, Ludwig Ctr Metastasis Res, Chicago, IL 60637 USA
来源：
ANTICANCER RESEARCH | 2013年 / 33卷 / 04期
关键词：
GFP; 53BP1; focus; UVC; DNA damage; pancreatic cancer; FLUORESCENT PROTEINS; GFP; EFFICACY; MOUSE; LIGHT;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We have previously demonstrated that ultraviolet (UV) light treatment is effective against various types of cancer cells expressing fluorescent proteins. In order to further understand the efficacy of UV treatment of cancer cells, we determined the kinetics of focus formation by imaging of a DNA damage-response (DDR) protein after UVC irradiation of human pancreatic cancer cells. A fusion protein consisting of the DDR protein 53BP1 and green fluorescent protein (GFP) (GFP-53BP1) was used as a live-cell imaging marker for cellular response after UVC irradiation. GFP-53BP1 foci were observed after UVC irradiation of MiaPaCa-2 human pancreatic cancer cells. During live-cell imaging, GFP-53BP1 foci were observed in the cells within 15 min after UVC irradiation, and some of the foci remained stable for at least three hours. GFP-53BP1 focus formation was observed in the pancreatic-cancer cells irradiated by 25-200,J/m(2) UVC. Our results indicate that an early response to DNA damage caused by UVC irradiation can be visualized by increased GFP-53BP1 focus formation by pancreatic cancer cells.
引用
收藏
页码：1373 / 1377
页数：5
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