Rapid isolation of cancer cells using microfluidic deterministic lateral displacement structure

被引：189
作者：
Liu, Zongbin [1 ]
Huang, Fei [1 ,2 ]
Du, Jinghui [1 ,3 ]
Shu, Weiliang [1 ]
Feng, Hongtao [1 ]
Xu, Xiaoping [1 ,4 ]
Chen, Yan [1 ]
机构：
[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China
[2] Sichuan Univ, Coll Chem Engn, Chengdu 610065, Peoples R China
[3] Nanchang Univ, Grad Sch, Dept Med, Nanchang 330006, Peoples R China
[4] Univ Hong Kong, Shenzhen Hosp, Shenzhen 518053, Peoples R China
来源：
BIOMICROFLUIDICS | 2013年 / 7卷 / 01期
基金：
中国国家自然科学基金;
关键词：
biomechanics; biomedical equipment; bioMEMS; blood; cancer; cellular biophysics; hydrodynamics; microfluidics; tumours; CIRCULATING TUMOR-CELLS; CAPTURE; ARRAYS; SIZE;
D O I：
10.1063/1.4774308
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This work reports a microfluidic device with deterministic lateral displacement (DLD) arrays allowing rapid and label-free cancer cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. Experiment data and theoretical simulation are presented to evaluate the isolation efficiency of various types of cancer cells in the microfluidic DLD structure. We also demonstrated the use of both circular and triangular post arrays for cancer cell separation in cell solution and blood samples. The device was able to achieve high cancer cell isolation efficiency and enrichment factor with our optimized design. Therefore, this platform with DLD structure shows great potential on fundamental and clinical studies of circulating tumor cells. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774308]
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页数：10
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