Comprehensive gene expression analysis by transcript profiling

被引：83

作者：

Donson, J ^{[1
]}

Fang, YW ^{[1
]}

Espiritu-Santo, G ^{[1
]}

Xing, WM ^{[1
]}

Salazar, A ^{[1
]}

Miyamoto, S ^{[1
]}

Armendarez, V ^{[1
]}

Volkmuth, W ^{[1
]}

机构：

[1] Ceres Inc, Malibu, CA 90265 USA

来源：

PLANT MOLECULAR BIOLOGY | 2002年 / 48卷 / 1-2期

关键词：

cDNA-AFLP; gene expression; microarray; oligonucleotide-based array; SAGE; transcript profiling;

D O I：

10.1023/A:1013722224489

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After the completion of the genomic sequence of Arabidopsis thaliana, it is now a priority to identify all the genes, their patterns of expression and functions. Transcript profiling is playing a substantial role in annotating and determining gene functions, having advanced from one-gene-at-a-time methods to technologies that provide a holistic view of the genome. In this review, comprehensive transcript profiling methodologies are described, including two that are used extensively by the authors, cDNA-AFLP and cDNA microarraying. Both these technologies illustrate the requirement to integrate molecular biology, automation, LIMS and data analysis. With so much uncharted territory in the Arabidopsis genome, and the desire to tackle complex biological traits, such integrated systems will provide a rich source of data for the correlative, functional annotation of genes.

引用

收藏

页码：75 / 97

页数：23

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