Body fluid identification in forensics

被引:141
作者
An, Ja Hyun [1 ]
Shin, Kyoung-Jin [1 ,2 ]
Yang, Woo Ick [1 ]
Lee, Hwan Young [1 ,2 ]
机构
[1] Yonsei Univ, Coll Med, Dept Forens Med, Seoul 120752, South Korea
[2] Yonsei Univ, Human Identificat Res Ctr, Seoul 120752, South Korea
基金
新加坡国家研究基金会;
关键词
Alternate light source; Body fluid identification; Catalytic test; Chemical test; DNA methylation; Forensic; Immunological test; miRNA; mRNA; PROSTATE-SPECIFIC ANTIGEN; DIFFERENTIALLY METHYLATED REGIONS; MESSENGER-RNA; DNA METHYLATION; MICRORNA EXPRESSION; BLOOD; SALIVA; STAINS; BIOGENESIS; GENETICS;
D O I
10.5483/BMBRep.2012.45.10.206
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Determination of the type and origin of the body fluids found at a crime scene can give important insights into crime scene reconstruction by supporting a link between sample donors and actual criminal acts. For more than a century, numerous types of body fluid identification methods have been developed, such as chemical tests, immunological tests, protein catalytic activity tests, spectroscopic methods and microscopy. However, these conventional body fluid identification methods are mostly presumptive, and are carried out for only one body fluid at a time. Therefore, the use of a molecular genetics-based approach using RNA profiling or DNA methylation detection has been recently proposed to supplant conventional body fluid identification methods. Several RNA markers and tDMRs (tissue-specific differentially methylated regions) which are specific to forensically relevant body fluids have been identified, and their specificities and sensitivities have been tested using various samples. In this review, we provide an overview of the present knowledge and the most recent developments in forensic body fluid identification and discuss its possible practical application to forensic casework. [BMB Reports 2012; 45(10): 545-553]
引用
收藏
页码:545 / 553
页数:9
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