Promoter-Dependent Translation Controlled by p54(nrb) and hnRNPM during Myoblast Differentiation

被引：19

作者：

Ainaoui, Nadera ^{[1
]}

Hantelys, Fransky ^{[1
]}

Renaud-Gabardos, Edith ^{[1
]}

Bunel, Morgane ^{[1
]}

Lopez, Frederic ^{[2
]}

Pujol, Francoise ^{[1
]}

Planes, Remi ^{[3
]}

Bahraoui, Elmostafa ^{[3
]}

Pichereaux, Carole ^{[4
]}

Burlet-Schiltz, Odile ^{[4
]}

Parini, Angelo ^{[5
]}

Garmy-Susini, Barbara ^{[5
]}

Prats, Anne-Catherine ^{[1
]}

机构：

[1] TRADGENE, UPS EA4554, Toulouse, France

[2] UPS, INSERM, UMR CRCT U1037, Toulouse, France

[3] UPS, INSERM, UMR CRCT U1043, Toulouse, France

[4] UPS, CNRS, UMR IPBS U5282, Toulouse, France

[5] UPS, CNRS, UMR I2MC U1048, Toulouse, France

来源：

PLOS ONE | 2015年 / 10卷 / 09期

关键词：

RIBOSOME ENTRY SITE; CAP-INDEPENDENT TRANSLATION; SPLICING REGULATORY PROTEIN; TRACT-BINDING-PROTEIN; TRANS-ACTING FACTORS; MEDIATED TRANSLATION; GENE-EXPRESSION; MESSENGER-RNA; BAG-1; IRES; IN-VITRO;

D O I：

10.1371/journal.pone.0136466

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fibroblast growth factor 1 (FGF1) is induced during myoblast differentiation at both transcriptional and translational levels. Here, we identify hnRNPM and p54(nrb)/NONO present in protein complexes bound to the FGF1 promoter and to the mRNA internal ribosome entry site (IRES). Knockdown or overexpression of these proteins indicate that they cooperate in activating IRES-dependent translation during myoblast differentiation, in a promoter-dependent manner. Importantly, mRNA transfection and promoter deletion experiments clearly demonstrate the impact of the FGF1 promoter on the activation of IRES-dependent translation via p54(nrb) and hnRNPM. Accordingly, knockdown of either p54 or hnRNPM also blocks endogenous FGF1 induction and myotube formation, demonstrating the physiological relevance of this mechanism and the role of these two proteins in myogenesis. Our study demonstrates the cooperative function of hnRNPM and p54(nrb) as regulators of IRES-dependent translation and indicates the involvement of a promoter-dependent mechanism.

引用

页数：25

共 53 条

[1] A search for structurally similar cellular internal ribosome entry sites [J].