Development of a reliable bioanalytical method based on prostate specific antigen trapping on the cavity of molecular imprinted polymer towards sensing of PSA using binding affinity of PSA-MIP receptor: A novel biosensor

In this study, electrically-conducting poly [Toluidine Blue (PTB)] was applied as artificial receptor. It was organized by molecular imprinting approaches and via electrochemical technique for the sensitive monitoring of prostate-specific antigen (PSA). The protein-imprinted PTB was electropolymerized in a pre-formed glutaraldehyde-cysteamine (GA-Cys A) matrix on the surface of gold electrode, which significantly boosted the stability against degradation of the Molecular Imprinted Polymer (MIP) on the surface of pre-modified gold electrode. Moreover, the MIP bio-receptor ability towards protein recognition was explored by some electrochemical techniques. The binding affinity of MIP system was considerably upper than that of non-imprinted polymer (NIP) system, indicating the success of the method in generating imprinted materials that was specifically use to PSA protein. The incubation of the MIP modified elec- trode in various concentration of PSA (from 1-60 mu g/L) resulted in the increase of the Fe (CN)(6)(3-/4-) redox peak current. The bio-device also showed linear response from 1-60 mu g/L, and LLOQ of 1 mu g/L, by using DPV technique, leading to PSA monitoring in clinical samples. The proposed MIP-based biosensor was satisfactorily applied to the determination of PSA in human plasma samples. Therefore, the developed bio-device provides a new approach for sensitive, simple, rapid, and cost-effective monitoring of 1 mu g/L, of PSA. Notably, this approach could appear as an appropriate candidate for point-of-care (POC) use in clinical and biomedical analyses. (C) 2020 Elsevier B.V. All rights reserved.