Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity

被引:61
作者
Shin, Won-Sub [1 ]
Lee, Bongsoo [1 ]
Jeong, Byeong-ryool [1 ]
Chang, Yong Keun [1 ,2 ]
Kwon, Jong-Hee [3 ]
机构
[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, 291 Daehak Ro, Daejeon 305701, South Korea
[2] Korea Adv Inst Sci & Technol, Adv Biomass R&D Ctr, 291 Daehak Ro, Daejeon 305701, South Korea
[3] Gyeongsang Natl Univ, Inst Agr & Life Sci, Dept Food Sci & Technol, Jinju 660701, South Korea
基金
新加坡国家研究基金会;
关键词
Microalgae; Light-harvesting chlorophyll antenna; Photosynthetic productivity; Chlorella vulgaris; ALGA CHLAMYDOMONAS-REINHARDTII; PHOTOSYSTEM-II; PHOTOSYNTHESIS; COMPLEX; PHOTOINHIBITION; ORGANIZATION; EXPRESSION; EFFICIENCY; CONVERSION; REDUCTION;
D O I
10.1007/s10811-016-0874-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microalgae have been proposed as eco-friendly feedstocks for biodiesel production, because they accumulate large amounts of lipids and increase their biomass through photosynthesis. However, the photosynthetic efficiency of microalgae is too low for this strategy to be economically feasible. In an effort to overcome this issue, random mutants with reduced chlorophyll antenna size were generated by ethyl methanesulfonate (EMS)-mediated mutagenesis of Chlorella vulgaris. The antenna size mutant, herein designated E5, exhibited 56.5 and 75.8 % decreases in chlorophyll a and b contents, respectively, with significant reductions in the expression levels of peripheral light-harvesting antenna proteins in photosystem II. The saturated photosynthetic activity and electron transport rate of the E5 mutant were significantly higher and also showed reduced non-photochemical quenching (NPQ), compared to those of the wild type. Consequentially, the E5 mutant cultures achieved 44.5 % improvement in biomass productivity under high light (200 mu mol photons m(-2) s(-1)). These results suggest that improving the photosynthetic efficiency of microalgae could greatly enhance their biomass production, and such mutant strains can be applicable for large-scale outdoor cultivation which is typically exposed to high light intensity.
引用
收藏
页码:3193 / 3202
页数:10
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