DETECTION OF ABNORMALITIES IN THE SUPERFICIAL ZONE OF CARTILAGE REPAIRED USING A TISSUE ENGINEERED CONSTRUCT DERIVED FROM SYNOVIAL STEM CELLS

被引:32
作者
Ando, Wataru [1 ,2 ,3 ]
Fujie, Hiromichi [4 ,5 ]
Moriguchi, Yu [1 ]
Nansai, Ryosuke [4 ]
Shimomura, Kazunori [1 ]
Hart, David A. [2 ]
Yoshikawa, Hideki [1 ]
Nakamura, Norimasa [1 ,6 ,7 ]
机构
[1] Osaka Univ, Grad Sch Med, Dept Orthopaed, Suita, Osaka 5650871, Japan
[2] Univ Calgary, Fac Med, McCaig Inst Bone & Joint Hlth, Calgary, AB, Canada
[3] Kansai Rosai Hosp, Dept Orthopaed, Amagasaki, Hyogo, Japan
[4] Kogakuin Univ, Dept Mech Engn, Biomech Lab, Hachioji, Tokyo, Japan
[5] Tokyo Metropolitan Univ, Fac Syst Design, Biomech Lab, Hino, Tokyo, Japan
[6] Osaka Univ, Ctr Adv Med Engn & Informat, Suita, Osaka 5650871, Japan
[7] Osaka Hlth Sci Univ, Inst Med Sci Sports, Osaka, Japan
关键词
Cartilage tissue engineering; animal model; image analysis; mechanical properties; mechanical test; microstructure;
D O I
10.22203/eCM.v024a21
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The present study investigated the surface structure and mechanical properties of repair cartilage generated from a tissue engineered construct (TEC) derived from synovial mesenchymal stem cells at six months post-implantation compared to those of uninjured cartilage. TEC-mediated repair tissue was cartilaginous with Safranin O staining, and had comparable macro-scale compressive properties with uninjured cartilage. However, morphological assessments revealed that the superficial zone of TEC-mediated tissue was more fibrocartilage-like, in contrast to the middle or deep zones that were more hyaline cartilage-like with Safranin O staining. Histological scoring of the TEC-mediated tissue was significantly lower in the superficial zone than in the middle and deep zones. Scanning electron microscopy showed a thick tangential bundle of collagen fibres at the most superficial layer of uninjured cartilage, while no corresponding structure was detected at the surface of TEC-mediated tissue. Immunohistochemical analysis revealed that PRG4 was localised in the superficial area of uninjured cartilage, as well as the TEC-mediated tissue. Friction testing showed that the lubrication properties of the two tissues was similar, however, micro-indentation analysis revealed that the surface stiffness of the TEC-repair tissue was significantly lower than that of uninjured cartilage. Permeability testing indicated that the TEC-mediated tissue exhibited lower water retaining capacity than did uninjured cartilage, specifically at the superficial zone. Thus, TEC-mediated tissue exhibited compromised mechanical properties at the superficial zone, properties which need improvement in the future for maintenance of long term repair cartilage integrity.
引用
收藏
页码:292 / 307
页数:16
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