Transgenic tobacco plants as production platform for biologically active human interleukin 2 and its fusion with proteinase inhibitors

被引:9
作者
Redkiewicz, Patrycja [1 ]
Wiesyk, Aneta [1 ]
Gora-Sochacka, Anna [1 ]
Sirko, Agnieszka [1 ]
机构
[1] Polish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland
关键词
human interleukin 2; fusion protein; proteinase inhibitor; transgenic plants; molecular farming; COLONY-STIMULATING FACTOR; SERINE PROTEINASES; RECOMBINANT PHARMACEUTICALS; SQUASH INHIBITORS; IMMUNE-RESPONSES; CUCURBITA-MAXIMA; HIV-INFECTION; CMTI-I; EXPRESSION; VACCINE;
D O I
10.1111/j.1467-7652.2012.00698.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Transgenic plants offer a low-cost approach for the production of pharmaceutically important and commercially valuable recombinant proteins. Our studies were focused on the plant-based production of human interleukin 2 (hIL-2) and its fusion with proteinase inhibitors, either SPI2 from Galleria mellonella or CMTI from Cucurbita maxima. Finally, five plant expression cassettes were obtained. Three of them contained the single cDNA encoding CMTI I, SPI2 and hIL-2, respectively, while two of them contained the translational fusion, SPI2::hIL-2 and CMTI::hIL-2. In all cases, the transgenes were controlled by the RbcS1 promoter and terminator and the recombinant proteins were targeted to the endoplasmic reticulum. After tobacco transformation, five groups of transgenic plants were obtained and analysed. The level of recombinant proteins was estimated either by Western blot or by ELISA. The biological activity of plant-produced hIL-2 alone or in a fusion with SPI2 or CMTI was confirmed using the mammalian cells proliferation assay. The activities of proteinase inhibitors were confirmed in proteolysis assay using azocoll as a substrate. The usefulness of using proteinase inhibitor CMTI I in a fusion with hIL-2 as a protective agent against trypsin digestion was demonstrated.
引用
收藏
页码:806 / 814
页数:9
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