Glutathione improves early somatic embryogenesis in Araucaria angustifolia (Bert) O. Kuntze by alteration in nitric oxide emission

被引：38

作者：

Vieira, Leila do Nascimento ^{[1
]}

Santa-Catarina, Claudete ^{[2
]}

de Freitas Fraga, Hugo Pacheco ^{[1
]}

Wendt dos Santos, Andre Luis ^{[3
]}

Steinmacher, Douglas Andre ^{[1
]}

Schlogl, Paulo Sergio ^{[1
]}

Silveira, Vanildo ^{[4
]}

Steinera, Neusa ^{[1
]}

Segal Floh, Eny Lochevet ^{[3
]}

Guerra, Miguel Pedro ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Lab Plant Dev Physiol & Genet, Grad Program Plant Genet Resources, BR-88040900 Florianopolis, SC, Brazil

[2] State Univ N Fluminense, Lab Tissue & Cellular Biol, CBB, BR-28013602 Rio De Janeiro, Brazil

[3] Univ Sao Paulo, Lab Plant Cell Biol, Dept Bot, IB, BR-05422970 Sao Paulo, Brazil

[4] State Univ N Fluminense, Biotechnol Lab, CBB, BR-28013602 Rio De Janeiro, Brazil

来源：

PLANT SCIENCE | 2012年 / 195卷

关键词：

Glutathione; Nitric oxide; Araucaria angustifolia; Conifers; Somatic embryogenesis; INTRACELLULAR GLUTATHIONE; SUSPENSION-CULTURES; PICEA-ABIES; POLYAMINE; GROWTH; NITROSYLATION; BIOSYNTHESIS; MATURATION; CHARCOAL; PROTEIN;

D O I：

10.1016/j.plantsci.2012.06.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, it was observed a straight relationship between the manipulation of the reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio, nitric oxide emission and quality and number of early somatic embryos in Araucaria angustifolia, a Brazilian endangered native conifer. In low concentrations GSH (0.01 and 0.1 mM) is a potential NO scavenger in the culture medium. Furthermore, it can increase the number of early SE formed in cell suspension culture media in a few days. However, the maintenance in this low redox state lead to a loss of early somatic embryos polarization. In gelled culture medium, high levels of GSH (5 mM) allows the development of globular embryos presenting a high NO emission on embryo apex, stressing its importance in the differentiation and cell division. Taken together these results indicate that the modification of the embryogenic cultures redox state might be an effective strategy to develop more efficient embryogenic systems in A. angustifolia. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：80 / 87

页数：8

共 44 条

[1] CHEMICAL MECHANISMS UNDERLYING THE VASODILATOR AND PLATELET ANTI-AGGREGATING PROPERTIES OF S-NITROSO-N-ACETYL-DL-PENICILLAMINE AND S-NITROSOGLUTATHIONE [J].