A Country-Wide Study of Spoligotype and Drug Resistance Characteristics of Mycobacterium tuberculosis Isolates from Children in China

被引：9

作者：

Jiao, Weiwei ^{[1
,2
]}

Liu, Zhiguang ^{[3
,4
]}

Han, Rui ^{[1
,2
]}

Zhao, Xiuqin ^{[3
,4
]}

Dong, Fang ^{[1
,2
]}

Dong, Haiyan ^{[3
,4
]}

Huang, Hairong ^{[5
]}

Tian, Jianling ^{[1
,2
]}

Li, Qinjing ^{[1
,2
]}

Lian, Lulu ^{[3
,4
]}

Yin, Qingqin ^{[1
,2
]}

Song, Wenqi ^{[1
,2
]}

Wan, Kanglin ^{[3
,4
]}

Shen, A-dong ^{[1
,2
]}

机构：

[1] Capital Med Univ, Beijing Childrens Hosp, Key Lab Major Dis Children, Minist Educ,Beijing Pediat Res Inst, Beijing, Peoples R China

[2] Capital Med Univ, Beijing Childrens Hosp, Natl Key Discipline Pediat, Minist Educ,Beijing Pediat Res Inst, Beijing, Peoples R China

[3] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing, Peoples R China

[4] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China

[5] Capital Med Univ, Beijing Chest Hosp, Natl TB Clin Lab, Beijing, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 12期

基金：

中国国家自然科学基金;

关键词：

BEIJING GENOTYPE; STRAINS; EPIDEMIOLOGY; PROVINCES;

D O I：

10.1371/journal.pone.0084315

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background: Tuberculosis (TB) is still a big threat to human health, especially in children. However, an isolation of Mycobacterium tuberculosis culture from pediatric cases remains a challenge. In order to provide some scientific basis for children TB control, we investigated the genotyping and drug resistance characteristics of M. tuberculosis isolates from pediatric cases in China. Methodology/Principal Findings: In this study, a total of 440 strains including 90 from children (<15 years), 159 from adolescents (15-18 years) and 191 from adults (>18 years) isolated in 25 provinces across China were subjected to spoligotyping and drug susceptibility testing. As a result, Beijing family strains were shown to remain predominant in China (85.6%, 81.1% and 75.4% in three above groups, respectively), especially among new children cases (91.0% vs. 69.6% in previously treated cases, P = 0.03). The prevalence of the Beijing genotype isolates was higher in northern and central China in the total collection (85.1% in northern and 83.9% in central vs. 61.6% in southern China, P<0.001) and a similar trend was seen in all three age groups (P = 0.708, <0.001 and 0.025, respectively). In adolescents, the frequencies of isoniazid (INH)-resistant and ethambutol (EMB)-resistant isolates were significantly higher among Beijing strains compared to non-Beijing genotype strains (P = 0.028 for INH and P = 0.027 for EMB). Furthermore, strong association was observed between resistance to rifampicine (RIF), streptomycin (STR) and multidrug resistance (MDR) among Beijing compared to non-Beijing strains in previously treated cases of children (P = 0.01, 0.01 and 0.025, respectively). Conclusion/Significance: Beijing family was more prevalent in northern and central China compared to southern China and these strains were predominant in all age groups. The genetic diversity of M. tuberculosis isolates from children was similar to that found in adolescents and adults. Beijing genotype was associated with RIF, STR and MDR resistance in previously treated children.

引用

页数：8

共 21 条

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