Bcl-2 and adenovirus E1B 19 kDA protein prevent E1A-induced processing of CPP32 and cleavage of poly(ADP-ribose) polymerase

被引:1
|
作者
Boulakia, CA
Chen, G
Ng, FWH
Teodoro, JG
Branton, PE
Nicholson, DW
Poirier, GG
Shore, GC
机构
[1] MCGILL UNIV,DEPT BIOCHEM,MONTREAL,PQ H3G 1Y6,CANADA
[2] MERCK FROSST CTR THERAPEUT RES,DEPT BIOCHEM & MOLEC BIOL,POINTE CLAIRE,PQ H9R 4P8,CANADA
[3] CHU LAVAL,RES CTR,DEPT MOLEC ENDOCRINOL,POLY ADP RIBOSE METAB GRP,ST FOY,PQ G1V 4G2,CANADA
关键词
apoptosia; E1A; PARP; CPP32; Bcl-2; E1B; 19K;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The E1A oncoproteins of adenovirus type 5 are potent inducers of apoptotic cell death. To manifest growth promoting and transforming properties, therefore, E1A requires the co-expression of a suppressor of apoptosis. During normal viral infection, this function is provided by the E1B 19 kDa protein. However, the cellular suppressor Bcl-2 can substitute for 19K during infection, and both proteins can effectively cooperate with E1A to facilitate transformation of primary cells in culture. How E1A induces apoptosis and at what point(s) on this pathway Bcl-2 and E1B 19K act are not presently known. Here, we demonstrate that E1A-induced apoptosis is accompanied by specific endo-proteolytic cleavage of poly(ADP-ribose) polymerase (PARP), an event that is linked to the Ced-3/ICE apoptotic pathway in other systems. PARP cleavage was also observed in p53-null cells infected with 19K(-) virus expressing 13S EIA. In addition to PARP cleavage, expression of E1A caused processing of the zymogen form of CPP32, a Ced-3/ICE protease that cleaves PARP and is required for apoptosis in mammalian cells. These events were prevented when EIA was co-expressed with E1B 19K or BCL-2, which places these suppressors of apoptosis either at or upstream of processing of pro-CPP32.
引用
收藏
页码:529 / 535
页数:7
相关论文
共 50 条
  • [1] Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly (ADP-ribose) polymerase and U1-70 kD proteins in staurosporine-mediated apoptosis
    Estoppey, S
    Rodriguez, I
    Sadoul, R
    Martinou, JC
    CELL DEATH AND DIFFERENTIATION, 1997, 4 (01): : 34 - 38
  • [2] Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly (ADP-ribose) polymerase and U1-70 kD proteins in staurosporine-mediated apoptosis
    Sandrine Estoppey
    Ivan Rodriguez
    Rémy Sadoul
    Jean-Claude Martinou
    Cell Death & Differentiation, 1997, 4 : 34 - 38
  • [3] Role of Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 in myocardial cells in diabetes
    Zhou, Wenzhong
    Yang, Jian
    Zhang, Di
    Li, Fengyin
    Li, Guo
    Gu, Yanyun
    Luo, Min
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2015, 10 (01) : 67 - 73
  • [4] THE ADENOVIRUS E1A PROTEINS INDUCE APOPTOSIS, WHICH IS INHIBITED BY THE E1B 19-KDA AND BCL-2 PROTEINS
    RAO, L
    DEBBAS, M
    SABBATINI, P
    HOCKENBERY, D
    KORSMEYER, S
    WHITE, E
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) : 7742 - 7746
  • [5] RELIEF OF P53-MEDIATED TRANSCRIPTIONAL REPRESSION BY THE ADENOVIRUS E1B 19-KDA PROTEIN OR THE CELLULAR BCL-2 PROTEIN
    SHEN, YQ
    SHENK, T
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (19) : 8940 - 8944
  • [6] Mutant Huntingtin induces activation of the Bcl-2/adenovirus E1B 19-kDa interacting protein (BNip3)
    Sassone, J.
    Colciago, C.
    Marchi, P.
    Ascardi, C.
    Alberti, L.
    Di Pardo, A.
    Zippel, R.
    Sipione, S.
    Silani, V.
    Ciammola, A.
    CELL DEATH & DISEASE, 2010, 1 : e7 - e7
  • [7] Ischemic preconditioning prevents ischemia/reperfusion-induced activation of protease CPP32 and cleavage of poly(ADP-ribose) polymerase in rat hearts in vivo
    Piot, CA
    Martini, JF
    Bui, SK
    Weiner, RI
    Wolfe, CL
    JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1998, 31 (02) : 448A - 448A
  • [8] CLONING OF A BCL-2 HOMOLOG BY INTERACTION WITH ADENOVIRUS E1B 19K
    FARROW, SN
    WHITE, JHM
    MARTINOU, I
    RAVEN, T
    PUN, KT
    GRINHAM, CJ
    MARTINOU, JC
    BROWN, R
    NATURE, 1995, 374 (6524) : 731 - 733
  • [9] Mutant Huntingtin induces activation of the Bcl-2/adenovirus E1B 19-kDa interacting protein (BNip3)
    J Sassone
    C Colciago
    P Marchi
    C Ascardi
    L Alberti
    A Di Pardo
    R Zippel
    S Sipione
    V Silani
    A Ciammola
    Cell Death & Disease, 2010, 1 : e7 - e7
  • [10] INDUCTION OF P53-INDEPENDENT APOPTOSIS BY HYGROMYCIN B - SUPPRESSION BY BCL-2 AND ADENOVIRUS E1B 19-KDA PROTEIN
    CHEN, G
    BRANTON, PE
    SHORE, GC
    EXPERIMENTAL CELL RESEARCH, 1995, 221 (01) : 55 - 59