Detection of Epstein-Barr Virus and Genotyping Based on EBNA2 Protein in Mexican Patients With Hodgkin Lymphoma: A Comparative Study in Children and Adults

被引：14

作者：

Palma, Icela ^{[1
,2
,3
]}

Elena Sanchez, Ana ^{[1
,2
,4
]}

Jimenez-Hernandez, Elva ^{[5
,6
]}

Alvarez-Rodriguez, Francisco ^{[5
]}

Nava-Frias, Margarita ^{[4
]}

Valencia-Mayoral, Pedro ^{[7
]}

Salinas-Lara, Citlatepet ^{[8
]}

Velazquez-Guadarrama, Norma ^{[4
]}

Portilla-Aguilar, Josefina ^{[5
]}

Pena, Rocio Y. ^{[9
]}

Ramos-Salazar, Patricia ^{[10
]}

Contreras, Alejandra ^{[11
]}

Alfaro, Ana ^{[12
]}

Maria Espinosa, Ana ^{[12
]}

Najera, Nayelli ^{[1
,2
]}

Gutierrez, Gisela ^{[13
,14
]}

Manuel Mejia-Arangure, Juan ^{[15
]}

Arellano-Galindo, Jose ^{[4
]}

机构：

[1] Inst Politecn Nacl, Escuela Super Med, Lab Morfol Celular, Plan De San Luis 11340, DF, Mexico

[2] Inst Politecn Nacl, Escuela Super Med, Lab Morfol Celular, Diaz Miron 11340, DF, Mexico

[3] Univ Nacl Autonoma Mexico, Dept Morfol, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico

[4] Hosp Infantil Mexico Dr Federico Gomez, Dept Infectol, Virol Lab, Mexico City 06720, DF, Mexico

[5] Hosp Pediat Moctezuma Secretaria Salud, Dept Oncol, Dept Dist Fed, Mexico City, DF, Mexico

[6] IMSS, Ctr Med Nacl la Raza, Dept Pediat Hematol, Unidad Med Alta Especialidad, Mexico City, DF, Mexico

[7] Hosp Infantil Mexico Dr Federico Gomez, Dept Patol, Mexico City 06720, DF, Mexico

[8] Inst Nacl Neurol & Neurocirugia Manuel Velasco Su, Dept Patol, Mexico City, DF, Mexico

[9] Inst Diagnost & Referenda Epidemiol Secretaria Sa, Mexico City 11800, DF, Mexico

[10] UMAE CMN la Raza IMSS, Dept Patol, Mexico City, DF, Mexico

[11] Hosp Infantil Mexico Dr Federico Gomez, Dept Biol Desarrollo, Mexico City 06720, DF, Mexico

[12] Hosp Gen Mexico City, Unidad Med Genom, Mexico City, DF, Mexico

[13] Inst Politecn Nacl, Escuela Super Med, Lab Med Regenerat, Plan De San Luis 11340, DF, Mexico

[14] Inst Politecn Nacl, Escuela Super Med, Lab Med Regenerat, Diaz Miron 11340, DF, Mexico

[15] IMSS, Mexico City, DF, Mexico

来源：

CLINICAL LYMPHOMA MYELOMA & LEUKEMIA | 2013年 / 13卷 / 03期

关键词：

DNA; EBER; EBNA2A; EBNA2B; Epstein-Barr virus; In situ hybridization; LMP; LYMPHOPROLIFERATIVE DISORDERS; EBV; DISEASE; GENE; REACTIVATION; PREVALENCE; EXPRESSION; LOCI;

D O I：

10.1016/j.clml.2012.11.010

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The frequency of Epstein-Barr virus (EBV) and the distribution of EBNA genotypes in children and adults with Hodgkin lymphoma (HL) in Mexico were analyzed. The frequency of EBV infection in patients previously diagnosed with lymphoma was high, was found most frequently in children rather than adults, and was more frequent in male patients than in female patients. EBNA2B was the most frequent genotype. Background: Epstein-Barr virus (EBV) is a member of the Herpesviridae family and is associated with Hodgkin lymphoma (HL). Isolates of EBV are classified according to sequence variation in the latency genes such as Epstein-Barr virus nuclear antigen (EBNA). EBNA2 contains the most divergent locus and is classified into type 1 and type 2 or EBNA2A and EBNA2B, respectively. We compared the frequency of EBV and the distribution of EBNA genotypes in Mexican children and adults with HL. Patients and Methods: Lymph node biopsy specimens from children and adults with HL were embedded in paraffin. EBV was identified by LMP1 amplification and Epstein-Barr-encoded RNA EBER by in situ hybridization (ISH) and genotyped as EBNA2A or EBNA2B using nested polymerase chain reaction (PCR) and specific primers for the detection of subtype. Results: Sixty-six samples were obtained from 3 hospitals-42 (63%) from children and 24 (37%) from adults with HL. Thirty-two of the 42 samples (76.1%) were positive for EBV in children and 16 of 24 (66.6%) samples were positive in adults (P = .41). In both children and adults, EBV was found more frequently in male patients. Thirty-four of 48 cases could be typed (70.8%). EBNA2A was found in 7/21 (33.3%) children and in 4/13 (30.8%) adults (P = 1.0), and EBNA2B was found in 10/21 (47.6%) children and in 9/13 (69.2%) adults (P = .22). A mix of subtypes was found in 4/21 (19%) children. Conclusion: EBV was found frequently in both children and adults with HL. EBNA2B was the most frequent subtype, and a high frequency of mixed subtypes was found in children. Published by Elsevier Inc.

引用

页码：266 / 272

页数：7

共 34 条

[1] Expression of Epstein-Barr virus in Hodgkin lymphoma in a population of United Arab Emirates nationals
Al-Salam, Suhail
John, Anne
Daoud, Sayel
Chong, Siew Ming
Castella, Antonio
[J]. LEUKEMIA & LYMPHOMA, 2008, 49 (09) : 1769 - 1777
[2] EPIDEMIOLOGIC EVIDENCE FOR THE 2-DISEASE HYPOTHESIS IN HODGKINS-DISEASE
ALEXANDER, FE
MCKINNEY, PA
WILLIAMS, J
RICKETTS, TJ
CARTWRIGHT, RA
[J]. INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 1991, 20 (02) : 354 - 361
[3] [Anonymous], 2011, ADV HEMATOL
[4] BELKAID MI, 1995, B CANCER, V82, P357
[5] BORISCH B, 1993, BLOOD, V82, P858
[6] Gammaherpesvirus and lymphoproliferative disorders in immunocompromised patients
Cesarman, Ethel
[J]. CANCER LETTERS, 2011, 305 (02) : 163 - 174
[7] COZEN W, 1992, CANCER EPIDEM BIOMAR, V1, P261
[8] Cellular factors associated with latency and spontaneous Epstein-Barr virus reactivation in B-lymphoblastoid cell lines
Davies, Michael L.
Xu, Shushen
Lyons-Weiler, James
Rosendorff, Adam
Webber, Steven A.
Wasil, Laura R.
Metes, Diana
Rowe, David T.
[J]. VIROLOGY, 2010, 400 (01) : 53 - 67
[9] High prevalence of a 30-base pair deletion in the Epstein-Barr virus (EBV) latent membrane protein 1 gene and of strain type B EBV in Mexican classical Hodgkin's disease and reactive lymphoid tissue
Dirnhofer, S
Angeles-Angeles, A
Ortiz-Hidalgo, C
Reyes, E
Gredler, E
Krugmann, J
Fend, F
Quintanilla-Martinez, L
[J]. HUMAN PATHOLOGY, 1999, 30 (07) : 781 - 787
[10] Racial/ethnic variation in EBV-positive classical Hodgkin lymphoma in California populations
Glaser, Sally L.
Gulley, Margaret L.
Clarke, Christina A.
Keegan, Theresa H.
Chang, Ellen T.
Shema, Sarah J.
Craig, Fiona E.
DiGiuseppe, Joseph A.
Dorfman, Ronald F.
Mann, Risa B.
Anton-Culver, Hoda
Ambinder, Richard F.
[J]. INTERNATIONAL JOURNAL OF CANCER, 2008, 123 (07) : 1499 - 1507

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