Total body irradiation tremendously impair the proliferation, differentiation and chromosomal integrity of bone marrow-derived mesenchymal stromal stem cells

被引：12

作者：

Lo, Wen-Jyi ^{[1
,2
]}

Lin, Chiao-Lin ^{[3
]}

Chang, Yu-Chien ^{[2
,3
]}

Bai, Li-Yuan ^{[1
,2
]}

Lin, Chen-Yuan ^{[2
]}

Liang, Ji-An ^{[4
]}

Li, Long-Yuan ^{[1
]}

Chao, Ling-Min ^{[2
]}

Chiu, Chang-Fang ^{[2
]}

Chen, Chuan-Mu ^{[1
]}

Yeh, Su-Peng ^{[2
,3
]}

机构：

[1] Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan

[2] China Med Univ, China Med Univ Hosp, Div Hematol & Oncol, Dept Internal Med, Taichung, Taiwan

[3] China Med Univ, China Med Univ Hosp, Dept Med Res, Stem Cell Res Lab, Taichung, Taiwan

[4] China Med Univ, China Med Univ Hosp, Dept Radiat Oncol, Taichung, Taiwan

来源：

ANNALS OF HEMATOLOGY | 2018年 / 97卷 / 04期

关键词：

Total body irradiation; Hematopoietic stem cell transplantation; Bone marrow-derived mesenchymal stromal stem cell; Chromosomal integrity; Radiation injury; SOLID CANCERS; HEMATOPOIETIC MICROENVIRONMENT; CHILDHOOD; TRANSPLANTATION; HYPERTRIGLYCERIDEMIA; TRANSFORMATION; COMPLICATIONS; PREVENTION; SURVIVORS; CAPACITY;

D O I：

10.1007/s00277-018-3231-y

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Total body irradiation (TBI) is frequently used in hematopoietic stem cell transplantation (HSCT) and is associated with many complications due to radiation injury to the normal cells, including normal stem cells. Nevertheless, the effects of TBI on the mesenchymal stromal stem cell (MSC) are not fully understood. Bone marrow-derived MSCs (BM-MSCs) isolated from normal adults were irradiated with 200 cGy twice daily for consecutive 3 days, a regimen identical to that used in TBI-conditioning HSCT. The characteristics, differentiation potential, cytogenetics, hematopoiesis-supporting function, and carcinogenicity of the irradiated BM-MSCs were then compared to the non-irradiated control. The irradiated and non-irradiated MSCs shared similar morphology, phenotype, and hematopoiesis-supporting function. However, irradiated MSCs showed much lower proliferative and differentiative potential. Irradiation also induced clonal cytogenetic abnormalities of MSCs. Nevertheless, the carcinogenicity of irradiated MSCs is low in vitro and in vivo. In parallel with the ex vivo irradiation experiments, decreased proliferative and differentiative abilities and clonal cytogenetic abnormalities can also be found in MSCs isolated from transplant recipients who had received TBI-based conditioning previously. Thus, TBI used in HSCT drastically injury MSCs and may contribute to the development of some long-term complications associated with clonal cytogenetic abnormality and poor adipogenesis and osteogenesis after TBI.

引用

页码：697 / 707

页数：11

共 47 条

[21] The effects of various irradiation doses on the growth and differentiation of marrow-derived human mesenchymal stromal cells [J].