Prevalence of Toxoplasma gondii from Free-Range Chickens (Gallus domesticus) from Addis Ababa, Ethiopia

被引：22

作者：

Tilahun, G. ^{[1
]}

Tiao, N. ^{[2
]}

Ferreira, L. R. ^{[3
]}

Choudhary, S. ^{[3
]}

Oliveira, S. ^{[3
]}

Verma, S. K. ^{[3
]}

Kwok, O. C. H. ^{[3
]}

Molla, B. ^{[2
]}

Saville, W. J. A. ^{[2
]}

Medhin, G. ^{[1
]}

Kassa, T. ^{[1
]}

Aleme, H. ^{[1
]}

Gebreyes, W. A. ^{[2
]}

Su, C. ^{[3
]}

Dubey, J. P. ^{[4
]}

机构：

[1] Univ Addis Ababa, Aklilu Lemma Inst Pathobiol, Addis Ababa, Ethiopia

[2] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA

[3] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA

[4] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

来源：

JOURNAL OF PARASITOLOGY | 2013年 / 99卷 / 04期

关键词：

D O I：

10.1645/12-25.1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Prevalence of Toxoplasma gondii in free-range chickens (Gallus domesticus) is a good indicator of the environmental contamination with oocysts because chickens become infected mainly by feeding from ground, feed, or soil contaminated with oocysts. The seroprevalence of T. gondii antibodies in 125 free-range chickens from the Addis Ababa, Ethiopia, was determined. Antibodies to T. gondii were assayed by the modified agglutination test; 48 of 125 (38.4%) chickens were seropositive, with titers of 1:5 in 14, 1:10 in 12, 1:20 in 14, 1:40 in 3, 1:80 in 1, 1:160 in 1, 1:320 in 1, and >= 1:640 in 2 chickens. The hearts of 115 chickens were bioassayed for T. gondii infection. Hearts of 72 seronegative (modified agglutination test [MAT] < 1:5) chickens were pooled in 4 groups (20 + 18 + 19 + 15) and fed to 4 T. gondii-free cats; none of these 4 cats shed oocysts in their feces examined 3-21 days after feeding chicken tissues. Hearts of 43 seropositive chickens (MAT >= 1:5) were bioassayed individually in mice. Toxoplasma gondii was isolated from only 1 chicken, with a MAT titer of 1:80. This isolate was designated TgCKEt1 and was not pathogenic for outbred mice. Restricted fragment length polymorphism (RFLP) genotyping using 10 loci indicated the TgCKEt1 was ToxoDB polymerase chain reaction-RFLP genotype #1 (Type II clonal). Results of this study indicate very low environmental contamination with T. gondii oocysts around Addis Ababa.

引用

页码：740 / 741

页数：2

共 9 条

[1] ISOLATION OF VIABLE TOXOPLASMA GONDII FROM TISSUES AND FECES OF CATS FROM ADDIS ABABA, ETHIOPIA [J].

Dubey, J. P. ;

Darrington, C. ;

Tiao, N. ;

Ferreira, L. R. ;

Choudhary, S. ;

Molla, B. ;

Saville, W. J. A. ;

Tilahun, G. ;

Kwok, O. C. H. ;

Gebreyes, W. A. .

JOURNAL OF PARASITOLOGY, 2013, 99 (01) :56-58

[2] A review of toxoplasmosis in humans and animals in Ethiopia [J].

Dubey, J. P. ;

Tiao, N. ;

Gebreyes, W. A. ;

Jones, J. L. .

EPIDEMIOLOGY AND INFECTION, 2012, 140 (11) :1935-1938

[3] Toxoplasma gondii Infections in Chickens (Gallus domesticus): Prevalence, Clinical Disease, Diagnosis and Public Health Significance [J].

Dubey, J. P. .

ZOONOSES AND PUBLIC HEALTH, 2010, 57 (01) :60-73

[4]

Dubey J.P., 1988, P1

[5] SEROLOGICAL RESPONSES OF EQUIDS FED TOXOPLASMA-GONDII OOCYSTS [J].

DUBEY, JP ;

DESMONTS, G .

EQUINE VETERINARY JOURNAL, 1987, 19 (04) :337-339

[6] INTERMEDIATE AND TRANSPORT HOSTS OF TOXOPLASMA-GONDII IN COSTA-RICA [J].

RUIZ, A ;

FRENKEL, JK .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1980, 29 (06) :1161-1166

[7] Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii [J].

Su, C. ;

Shwab, E. K. ;

Zhou, P. ;

Zhu, X. Q. ;

Dubey, J. P. .

PARASITOLOGY, 2010, 137 (01) :1-11

[8] Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages [J].

Su, Chunlei ;

Khan, Asis ;

Zhou, Peng ;

Majumdar, Debashree ;

Ajzenberg, Daniel ;

Darde, Marie-Laure ;

Zhu, Xing-Quan ;

Ajioka, James W. ;

Rosenthal, Benjamin M. ;

Dubey, Jitender P. ;

Sibley, L. David .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (15) :5844-5849

[9] An investigation into the seroprevalence of Toxoplasma gondii, Bartonella spp., feline immunodeficiency virus (FIV), and feline leukaemia virus (FeLV) in cats in Addis Ababa, Ethiopia [J].

Tiao, N. ;

Darrington, C. ;

Molla, B. ;

Saville, W. J. A. ;

Tilahun, G. ;

Kwok, O. C. H. ;

Gebreyes, W. A. ;

Lappin, M. R. ;

Jones, J. L. ;

Dubey, J. P. .

EPIDEMIOLOGY AND INFECTION, 2013, 141 (05) :1029-1033

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