Matched Placental and Circulating Plasmodium falciparum Parasites are Genetically Homologous at the var2csa ID1-DBL2X Locus by Deep Sequencing

被引：1

作者：

Waltmann, Andreea ^{[1
]}

Patel, Jaymin C. ^{[2
]}

Thwai, Kyaw L. ^{[2
]}

Hathaway, Nicholas J. ^{[3
]}

Parobek, Christian M. ^{[4
]}

Massougbodji, Achille ^{[5
]}

Fievet, Nadine ^{[6
,8
,9
]}

Bailey, Jeffery A. ^{[3
,7
]}

Deloron, Philippe ^{[6
,10
]}

Juliano, Jonathan J. ^{[1
,2
,4
]}

Ndam, Nicaise Tuikue ^{[6
,8
]}

Meshnick, Steven R. ^{[1
,2
]}

机构：

[1] Univ N Carolina, Inst Global Hlth & Infect Dis, Sch Med, Chapel Hill, NC USA

[2] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA

[3] Univ Massachusetts, Program Bioinformat & Integrat Biol, Worcester, MA 01605 USA

[4] Univ N Carolina, Curriculum Genet & Mol Biol, Chapel Hill, NC USA

[5] Univ Abomey Calavi, Ctr Etud & Rech Paludisme Associe Grossesse & Enf, Cotonou, Benin

[6] Univ Paris 05, COMUE Sorbonne Paris Cite, Inst Rech Dev, MERIT UMR216, Paris, France

[7] Univ Massachusetts, Div Transfus Med, Dept Med, Worcester, MA 01605 USA

[8] Inst Rech Dev, UMR 216, Paris, France

[9] Fac Sci Sante, Lab Parasitol, Cotonou, Benin

[10] Inst Rech Dev, UR010, Fac Pharm, Paris, France

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2018年 / 98卷 / 01期

基金：

美国国家卫生研究院;

关键词：

PREGNANCY-ASSOCIATED MALARIA; UMBILICAL-CORD BLOOD; INFECTED ERYTHROCYTES; CHONDROITIN SULFATE; BIRTH-WEIGHT; VAR GENE; WOMEN; BURDEN; QUANTIFICATION; POPULATIONS;

D O I：

10.4269/ajtmh.17-0529

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

In pregnancy-associated malaria, infected erythrocytes accumulate in the placenta. It is unclear if in polyclonal infections this results in distinct peripheral and placental parasite populations. We used long amplicon deep sequencing of Plasmodium falciparum var2csa ID1-DBL2X from 15 matched peripheral and placental samples collected at delivery from a high transmission area to determine genetic homology. Despite substantial sequence variation and detecting 23 haplotypes, the matched pairs mostly contained the same genetic variants, with 11 pairs sharing 100% of their variants, whereas others showed some heterogeneity. Thus, at delivery, peripheral and placental parasites appear to intermix and placental genotypes can be inferred through peripheral sampling.

引用

页码：77 / 82

页数：6

共 33 条

[31] Identification of VAR2CSA Domain-Specific Inhibitory Antibodies of the Plasmodium falciparum Erythrocyte Membrane Protein 1 Using a Novel Flow Cytometry Assay [J].

Obiakor, Harold ;

Avril, Marion ;

MacDonald, Nicholas J. ;

Srinivasan, Prakash ;

Reiter, Karine ;

Anderson, Charles ;

Holmes, Kevin L. ;

Fried, Michal ;

Duffy, Patrick E. ;

Smith, Joseph D. ;

Narum, David L. ;

Miller, Louis H. .

CLINICAL AND VACCINE IMMUNOLOGY, 2013, 20 (03) :433-442

[32] A Plasma Survey Using 38 PfEMP1 Domains Reveals Frequent Recognition of the Plasmodium falciparum Antigen VAR2CSA among Young Tanzanian Children [J].

Oleinikov, Andrew V. ;

Voronkova, Valentina V. ;

Frye, Isaac Tyler ;

Amos, Emily ;

Morrison, Robert ;

Fried, Michal ;

Duffy, Patrick E. .

PLOS ONE, 2012, 7 (01)

[33] Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain [J].

Nunes-Silva, Sofia ;

Gangnard, Stephane ;

Vidal, Marta ;

Vuchelen, Anneleen ;

Dechavanne, Sebastien ;

Chan, Sherwin ;

Pardon, Els ;

Steyaert, Jan ;

Ramboarina, Stephanie ;

Chene, Arnaud ;

Gamain, Benoit .

SCIENTIFIC REPORTS, 2014, 4

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