Multilocus sequence typing of Scedosporium apiospermum and Pseudallescheria boydii isolates from cystic fibrosis patients

被引：40

作者：

Bernhardt, A. ^{[1
]}

Sedlacek, L. ^{[2
]}

Wagner, S. ^{[3
]}

Schwarz, C. ^{[4
]}

Wuerstl, B. ^{[5
]}

Tintelnot, K. ^{[1
]}

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

[2] Hannover Med Sch, Inst Med Mikrobiol & Hyg, D-30625 Hannover, Germany

[3] HELIOS Klinikum Emil von Behring, D-14165 Berlin, Germany

[4] Charite, Christiane Herzog Zentrum Mukoviszidose, D-13353 Berlin, Germany

[5] Univ Munich, Max Von Pettenkofer Inst, D-80336 Munich, Germany

来源：

JOURNAL OF CYSTIC FIBROSIS | 2013年 / 12卷 / 06期

关键词：

Pseudallescheria boydii; Scedosporium apiospermum; Cystic fibrosis; MLST; Genotyping; SPECIES COMPLEX; COLONIZATION; SCHEME; PCR;

D O I：

10.1016/j.jcf.2013.05.007

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background: Scedosporium and Pseudallescheria species are the second most common lung-colonising fungi in cystic fibrosis (CF) patients. For epidemiological reasons it is important to trace sources of infection, routes of transmission and to determine whether these fungi are transient or permanent colonisers of the respiratory tract. Molecular typing methods like multilocus sequence typing (MLST) help provide this data. Methods: Clinical isolates of the P. boydii complex (including S. apiospermum and P. boydii) from CF patients in different regions of Germany were studied using MLST. Five gene loci, ACT, CAL, RPB2, BT2 and SOD2, were analysed. Results: The S. apiospermum isolates from 34 patients were assigned to 32 sequence types (STs), and the P. boydii isolates from 14 patients to 8 STs. The results revealed that patients can be colonised by individual strains for years. Conclusions: The MLST scheme developed for S. apiospermum and P. boydii is a highly effective tool for epidemiologic studies worldwide. The MLST data are accessible at http://mlst.mycologylab.org/. (C) 2013 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

引用

页码：592 / 598

页数：7

共 27 条

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