Highly permissive subclone of the porcine kidney cell line for porcine circovirus type 2 production

被引：10

作者：

Chen, Hsu-Chung Gabriel ^{[1
]}

Kuo, Tsun-Yung ^{[2
]}

Yang, Ying-Chen ^{[2
]}

Wu, Chung-Chin ^{[3
]}

Lai, Shiow-Suey ^{[1
]}

机构：

[1] Natl Taiwan Univ, Sch Vet Med, Taipei 10617, Taiwan

[2] Natl Ilan Univ, Dept Biotechnol & Anim Sci, Ilan 26041, Taiwan

[3] SBC Virbac Biotech Co Ltd, Taipei 11493, Taiwan

来源：

JOURNAL OF VIROLOGICAL METHODS | 2013年 / 187卷 / 02期

关键词：

Porcine circovirus type 2 (PCV2); Porcine kidney-15 (PK-15); Cell cloning; Cytopathic effect (CPE); ENHANCED REPLICATION; PCR ASSAY; VIRUS; SWINE;

D O I：

10.1016/j.jviromet.2012.11.013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study established a highly permissive and decontaminated cell line for growing porcine circovirus type 2 (PCV2). A porcine kidney-15 cell line (PK-15) contaminated with porcine circovirus type 1 (PCV1) was decontaminated by neutralizing with rabbit anti-PCV1 hyperimmune serum. Subsequently, by limiting dilution and cell subcloning, four PCV1-free monoclonal cells were grown to monolayers. Each cell clone and PK-15 cell were infected with PCV2. The PKKC cell clone yielded up to 10(6.8) TCID50/ml at 6 days post-infection. In addition, PKKC was free of extraneous viral contamination and exhibited a cytopathic effect (CPE) to PCV2 at 6 days post-infection. The advantages of the PKKC cell are that it can grow a high PCV2 titer and exhibit CPE; therefore, it can be used for PCV2 cultivation, vaccine production, and diagnostic purposes. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：380 / 383

页数：4

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