ATP dependence of the non-specific ion channel in Torpedo synaptic vesicles

被引：4

作者：

Ahdut-Hacohen, Ronit ^{[1
]}

Meiri, Halina ^{[1
]}

Rahamimoff, Rami ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Physiol, IL-91120 Jerusalem, Israel

来源：

NEUROREPORT | 2006年 / 17卷 / 06期

关键词：

ATP ion channels; transmitter release;

D O I：

10.1097/00001756-200604240-00019

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Synaptic vesicles of Torpedo electromotor neurons contain a high amount of ATP. The concentration of total ATP is around 120 mM, whereas the free [ATP] is about S-6 mM. We examined the effect of intravesicular ATP on the non-specific ion channel in Torpedo-fused synaptic vesicles. It was found that this channel is closed when the ATP concentration is above 2 mM, but it is very frequently open at lower ATP concentrations. Unmasking this ion channel at a low ATP concentration may be significant for post-fusion control of transmitter release by the 'kiss and run' mechanism in normal conditions, while during metabolic stress it may underlie dissipation of important gradients across the vesicle membrane.

引用

页码：653 / 656

页数：4

共 25 条

[1] Hydrogen ions control synaptic vesicle ion channel activity in Torpedo electromotor neurones [J].

Ahdut-Hacohen, R ;

Duridanova, D ;

Meiri, H ;

Rahamimoff, R .

JOURNAL OF PHYSIOLOGY-LONDON, 2004, 556 (02) :347-352

[2] Macula densa cell signaling involves ATP release through a maxi anion channel [J].

Bell, PD ;

Lapointe, JY ;

Sabirov, R ;

Hayashi, S ;

Peti-Peterdi, J ;

Manabe, K ;

Kovacs, G ;

Okada, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :4322-4327

[3] Purinergic signalling: ATP release [J].

Bodin, P ;

Burnstock, G .

NEUROCHEMICAL RESEARCH, 2001, 26 (8-9) :959-969

[4] VESICLE HYPOTHESIS OF THE RELEASE OF QUANTA OF ACETYLCHOLINE [J].

CECCARELLI, B ;

HURLBUT, WP .

PHYSIOLOGICAL REVIEWS, 1980, 60 (02) :396-441

[5] The insulin secretory granule is the major site of KATP channels of the endocrine pancreas [J].

Geng, XH ;

Li, LH ;

Watkins, S ;

Robbins, PD ;

Drain, P .

DIABETES, 2003, 52 (03) :767-776

[6] EXTRACELLULAR ATP - EFFECTS, SOURCES AND FATE [J].

GORDON, JL .

BIOCHEMICAL JOURNAL, 1986, 233 (02) :309-319

[7] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[8] NUCLEOTIDE-METABOLISM AND CELLULAR-DAMAGE IN MYOCARDIAL ISCHEMIA [J].

JENNINGS, RB ;

STEENBERGEN, C .

ANNUAL REVIEW OF PHYSIOLOGY, 1985, 47 :727-749

[9] STRUCTURE AND FUNCTION OF FUSION PORES IN EXOCYTOSIS AND ECTOPLASMIC MEMBRANE-FUSION [J].

LINDAU, M ;

ALMERS, W .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (04) :509-517

[10] REDUCED ATP CONCENTRATION AS A BASIS FOR SYNAPTIC TRANSMISSION FAILURE DURING HYPOXIA IN THE INVITRO GUINEA-PIG HIPPOCAMPUS [J].

LIPTON, P ;

WHITTINGHAM, TS .

JOURNAL OF PHYSIOLOGY-LONDON, 1982, 325 (APR) :51-65

← 1 2 3 →